| Author | Fonseca, Erica L. | |
| Author | Marin, Michel A. | |
| Author | Encinas, Fernando | |
| Author | Vicente, Ana Carolina P. | |
| Access date | 2016-03-29T15:23:57Z | |
| Available date | 2016-03-29T15:23:57Z | |
| Document date | 2015 | |
| Citation | FONSECA, Erica L. et al. Full characterization of the integrative and conjugative element carrying the metallo-b-lactamase blaSPM-1 and bicyclomycin bcr1 resistance genes found in the pandemic Pseudomonas aeruginosa clone SP/ST277. Journal of Antimicrobial Chemotherapy, v.70, n.9, p.2547 –2550, 2015. | pt_BR |
| ISSN | 0305-7453 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13387 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Pseudomonas aeruginosa | pt_BR |
| Title | Full characterization of the integrative and conjugative element carrying the metallo-b-lactamase blaSPM-1 and bicyclomycin bcr1 resistance genes found in the pandemic Pseudomonas aeruginosa clone SP/ST277 | pt_BR |
| Type | Article | |
| DOI | 10.1093/jac/dkv152 | |
| Abstract | Objectives: This study aimed to characterize the genomic context of the blaSPM-1 gene in Brazilian strains belonging
to the pandemic Pseudomonas aeruginosa clone SP/ST277.
Methods: WGS of clone SP/ST277 strains was performed using a Nextera paired-end library in an Illumina HiSeq
2500 sequencer. blaSPM-1 context was assessed by de novo assembly and gene prediction and annotation tools.
blaSPM-1 was screened in P. aeruginosa genomes through BlastN, and comparative genomics were performed.
Results: The metallo-b-lactamase blaSPM-1 has been disseminated by the pandemic Brazilian P. aeruginosa clone
SP/ST277. In spite of its association with the CR4 element and with the Tn4371 element, the overall blaSPM-1 genomic
context remains uncharacterized and its determination is valuable to understanding gene dispersion
dynamics and the consequent emergence of carbapenem resistance. In this study, blaSPM-1 and its surrounding
sequences (CR4-groEL-blaSPM-1-CR4-groEL) were found in the variable region of an ICE-like element resembling
Tn4371 (where ICE stands for integrative and conjugative element). This element, named ICETn43716061, had 46
ORFs, including the bicyclomycin resistance bcr1 gene. An integrase gene and a set of conjugative transfer genes
were identified. Gene content and order were shared with other Tn4371-ICEs, presenting remarkable amino acid
identities. blaSPM-1 and surrounding sequences were missing in ICETn43716061 of PS600-MA, another isolate
belonging to clone SP/ST277, indicating their mobilization. Eight/nine P. aeruginosa genomes assigned to clone
SP/ST277, by in silico MLST, harboured blaSPM-1 inserted into ICETn43716061.
Conclusions: The presence of blaSPM-1 in a Tn4371-ICE with intact integration/conjugation modules demonstrated
that, besides gene dispersion by clonal expansion of the pandemic SP/ST277 lineage, blaSPM-1 may be
spread through ICE conjugation. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | blaSPM-1 gene | pt_BR |
| Subject | bicyclomycin bcr1 resistance genes | pt_BR |
| Subject | pandemic SP/ST277 lineage | pt_BR |
| e-ISSN | 1460-2091 | |
