| Author | Wu, Ângela Cardoso de Alvarenga | |
| Author | Freitas, Michelle Aparecida Ribeiro de | |
| Author | Silva, Soraia de O | |
| Author | Nogueira, Paula Monalisa | |
| Author | Soares, Rodrigo Pedro Pinto | |
| Author | Pesquero, João Bosco | |
| Author | Gomes, Maria Aparecida | |
| Author | Pesquero, Jorge Luiz | |
| Author | Melo, Maria Norma | |
| Access date | 2016-04-01T18:16:53Z | |
| Available date | 2016-04-01T18:16:53Z | |
| Document date | 2015 | |
| Citation | WU, Ângela Cardoso de Alvarenga et al. Genetic differences between two Leishmania major-like strains revealed by suppression subtractive hybridization. Molecular and Biochemical Parasitology, Vol. 203, n. 1–2, p. 34–38, 2015 | pt_BR |
| ISSN | 0166-6851 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13439 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Ltd | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Genetic differences between two Leishmania major-like strains revealed by suppression subtractive hybridization | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.molbiopara.2015.10.005 | |
| Abstract | Leishmania major, the causative agent of zoonotic leishmaniasis, is restricted to Old World countries. Molecular and biochemical techniques have been used to identify some L. major-like isolated in South America including Brazil. Here, two L. major-like strains, one virulent (BH49) and one non-virulent (BH121), were subjected to suppression subtractive hybridization (SSH) technique in order to identify differentially expressed genes. SSH technique identified nine cDNA fragments exhibiting high homology to previously sequenced L. major genes. Five cDNAs (four specific for BH49 and one for BH121) were confirmed by RT-PCR. Among those differentially expressed subtracted genes, some were involved in physiological processes including metabolism, translation and destination of proteins, production of energy, virulence factors and unknown functions. Western-blot analysis confirmed a higher expression level of β-1,3-galactosyl residues in L. major-like lipophosphoglycan (LPG). This molecular analysis opens the possibility for identification of potential virulence factors not only in different strains, but also in others species of Leishmania. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Uberlândia. Instituto de Ciências Biomédicas. Instituto de Ciências Biológicas. Departamento de Fisiologia e Biofísica. Uberlândia, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Biofísica. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brasil | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil | pt_BR |
| Subject | Leishmania major-like | pt_BR |
| Subject | Virulence | pt_BR |
| Subject | Differential gene expression | pt_BR |
| Subject | Lipophosphoglycan | pt_BR |
| Subject | Phosphoglycan β-1,3-galactosyltransferase | pt_BR |
