Author | Rodríguez, Hugo Oswaldo Valdivia | |
Author | Scholte, Larissa Lopes Silva | |
Author | Oliveira, Guilherme Corrêa de | |
Author | Gabaldón, Toni | |
Author | Bartholomeu, Daniella Castanheira | |
Access date | 2016-04-01T19:15:38Z | |
Available date | 2016-04-01T19:15:38Z | |
Document date | 2015 | |
Citation | RODRÍGUEZ, Hugo Oswaldo Valdivia et al. The Leishmania metaphylome: A comprehensive survey of Leishmania protein phylogenetic relationships. BMC Genomics, vol. 16, p. 887, 2015 | pt_BR |
ISSN | 1471-2164 | |
URI | https://www.arca.fiocruz.br/handle/icict/13442 | |
Language | eng | pt_BR |
Publisher | BioMed Central | pt_BR |
Rights | open access | pt_BR |
Title | The Leishmania metaphylome: A comprehensive survey of Leishmania protein phylogenetic relationships | pt_BR |
Type | Article | pt_BR |
DOI | 10.1186/s12864-015-2091-2 | |
Abstract | Background: Leishmaniasis is a neglected parasitic disease with diverse clinical manifestations and a complex epidemiology. It has been shown that its parasite-related traits vary between species and that they modulate infectivity, pathogenicity, and virulence. However, understanding of the species-specific adaptations responsible for these features and their evolutionary background is limited. To improve our knowledge regarding the parasite biology and adaptation mechanisms of different Leishmania species, we conducted a proteome-wide phylogenomic analysis to gain insights into Leishmania evolution.
Results: The analysis of the reconstructed phylomes (totaling 45,918 phylogenies) allowed us to detect genes that are shared in pathogenic Leishmania species, such as calpain-like cysteine peptidases and 3'a2rel-related proteins, or genes that could be associated with visceral or cutaneous development. This analysis also established the phylogenetic relationship of several hypothetical proteins whose roles remain to be characterized. Our findings demonstrated that gene duplication constitutes an important evolutionary force in Leishmania, acting on protein families that mediate host-parasite interactions, such as amastins, GP63 metallopeptidases, cathepsin L-like proteases, and our methods permitted a deeper analysis of their phylogenetic relationships.
Conclusions: Our results highlight the importance of proteome wide phylogenetic analyses to detect adaptation and evolutionary processes in different organisms and underscore the need to characterize the role of expanded and species-specific proteins in the context of Leishmania evolution by providing a framework for the phylogenetic relationships of Leishmania proteins. | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Imunologia e Genômica de Parasitos. Belo Horizonte, MG, Brasil/Department of Parasitology, U.S. Naval Medical Research Unit No. 6. Lima, Peru/Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales. Lima, Peru. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Grupo de Genomica e Biologia Computacional. Belo Horizonte, MG, Brasil/Instituto Tecnológico Vale. Belém, PA, Brasil. | pt_BR |
Affilliation | Centre for Genomic Regulation. Bioinformatics and Genomics Programme. Barcelona, Spain/Universitat Pompeu Fabra. Barcelona, Spain/Institució Catalana de Recerca i Estudis Avançats. Barcelona, Spain | pt_BR |
Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Imunologia e Genômica de Parasitos. Belo Horizonte, MG, Brasil/Centro de Investigaciones Tecnológicas, Biomédicas y Medioambientales. Lima, Peru. | pt_BR |
Subject | Phylogenomics | pt_BR |
Subject | Leishmania | pt_BR |
Subject | Homology | pt_BR |
Subject | prediction | pt_BR |