| Author | Vivarini, Aislan de Carvalho | |
| Author | Pereira, Renata de Meirelles Santos | |
| Author | Souza, Victor Barreto de | |
| Author | Temerozo, Jairo Ramos | |
| Author | Soares, Deivid C. | |
| Author | Saraiva, Elvira M. | |
| Author | Saliba, Alessandra Mattos | |
| Author | Bou-Habib, Dumith Chequer | |
| Author | Lopes, Ulisses Gazos | |
| Access date | 2016-04-05T13:55:25Z | |
| Available date | 2016-04-05T13:55:25Z | |
| Document date | 2015 | |
| Citation | VIVARINI, Aislan de Carvalho; et al. HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR. Scientific Reports, v.5:16777, 11p, Nov. 2015. | pt_BR |
| ISSN | 2045-2322 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13529 | |
| Language | eng | pt_BR |
| Publisher | Nature Publishing Group | pt_BR |
| Rights | open access | |
| Title | HIV-1 Tat protein enhances the intracellular growth of Leishmania amazonensis via the ds-RNA induced protein PKR | pt_BR |
| Type | Article | |
| DOI | 10.1038/srep16777 | |
| Abstract | HIV-1 co-infection with human parasitic diseases is a growing public health problem worldwide. Leishmania parasites infect and replicate inside macrophages, thereby subverting host signaling pathways, including the response mediated by PKR. The HIV-1 Tat protein interacts with PKR and plays a pivotal role in HIV-1 replication. This study shows that Tat increases both the expression and activation of PKR in Leishmania-infected macrophages. Importantly, the positive effect of Tat addition on parasite growth was dependent on PKR signaling, as demonstrated in PKR-deficient macrophages or macrophages treated with the PKR inhibitor. The effect of HIV-1 Tat on parasite growth was prevented when the supernatant of HIV-1-infected macrophages was treated with neutralizing anti-HIV-1 Tat prior to Leishmania infection. The addition of HIV-1 Tat to Leishmania-infected macrophages led to inhibition of iNOS expression, modulation of NF-kB activation and enhancement of IL-10 expression. Accordingly, the expression of a Tat construct containing mutations in the basic region (49-57aa), which is responsible for the interaction with PKR, favored neither parasite growth nor IL-10 expression in infected macrophages. In summary, we show that Tat enhances Leishmania growth through PKR signaling. | pt_BR |
| Affilliation | Universidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Janeiro. Instituto de Microbiologia Paulo Góes. Laboratório de Imunobiologia de Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Janeiro. Instituto de Microbiologia Paulo Góes. Laboratório de Imunobiologia de Leishmanioses. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Departamento de Microbiologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Janeiro. Centro de Ciências da Saúde. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | HIV-1 | pt_BR |
| Subject | Tat protein | pt_BR |
| Subject | Leishmania amazonensis | pt_BR |
| Subject | protein PKR | pt_BR |
| Subject | ds-RNA | pt_BR |
| DeCS | HIV-1 | pt_BR |
| DeCS | Produtos do Gene tat | pt_BR |
| DeCS | Leishmania braziliensis | pt_BR |
| DeCS | eIF-2 Quinase | pt_BR |
