| Author | Morales, Bernardina Penarrieta | |
| Author | Trilles, Luciana | |
| Author | Bertho, Álvaro Luiz | |
| Author | Neves Junior, Ivan | |
| Author | Oliveira, Raquel de Vasconcellos Carvalhaes de | |
| Author | Wanke, Bodo | |
| Author | Lazéra, Márcia dos Santos | |
| Access date | 2016-04-07T13:49:03Z | |
| Available date | 2016-04-07T13:49:03Z | |
| Document date | 2015 | |
| Citation | MORALES, Bernardina Penarrieta; et al. In vitro susceptibility testing of amphotericin B for Cryptococcus neoformans variety grubii AFLP1/VNI and Cryptococcus gattii AFLP6/ VGII by CLSI and flow cytometry. Mycoses, v.58, p.273-279, 2015. | pt_BR |
| ISSN | 0933-7407 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13637 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Title | In vitro susceptibility testing of amphotericin B for Cryptococcus neoformans variety grubii AFLP1/VNI and Cryptococcus gattii AFLP6/ VGII by CLSI and flow cytometry | pt_BR |
| Type | Article | |
| DOI | 10.1111/myc.12310 | |
| Abstract | Cryptococcus neoformans var. grubii AFLP1/VNI is the main causative agent of cryptococcosis
associated with AIDS in the world. Cryptococcus gattii AFLP6/VGII causes
mainly endemic primary infection in immunocompetent hosts. To determine the
minimum inhibitory concentrations (MICs) of C. neoformans var. grubii AFLP1/VNI
and C. gattii AFLP6/VGII against amphotericin B (AMB) in a short period of time,
flow cytometry (FCM) with FUN-1 fluorochrome was used to compare with broth
microdilution method (CLSI M27-A3). The minimum incubation period was evaluated
by minimum fungicidal concentration procedure. Seventeen clinical isolates of
C. neoformans var. grubii AFLP1/VNI and 18 of C. gattii AFLP6/VGII were analysed.
The time for the determination of MICs by FCM was 2 h against 72 h by CLSI M27-
A3 and the comparison of MIC showed a positive significant correlation (P = 0.048).
It is important to highlight the role of the FCM as an alternative method to determine
the MICs for AMB in within a day, with positive cost-benefit. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Departamento de Epidemiologia e Estatística. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Doenças Infecciosas Evandro Chagas. Laboratório de Micologia Médica. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Cryptococcus neoformans | pt_BR |
| Subject | Cryptococcus gattii | pt_BR |
| Subject | amphotericin B | pt_BR |
| Subject | CLSI M27-A3 | pt_BR |
| Subject | Flow cytometry | pt_BR |
| DeCS | Cryptococcus neoformans | pt_BR |
| DeCS | Citometria de Fluxo | pt_BR |
| DeCS | Cryptococcus gattii | pt_BR |
| DeCS | Anfotericina B | pt_BR |
| e-ISSN | 1439-0507 | |
