| Author | Couto, Luzinei da Silva | |
| Author | Romão, Raquel Peralva Ribeiro | |
| Author | Saavedra, Andrea Franco | |
| Author | Souza, Beatriz Lilian da Silva Costa | |
| Author | Moreira, Otacilio Cruz | |
| Author | Silva, Adriano Gomes | |
| Author | Bergmann, Bartira Rossi | |
| Author | Cruz, Alda Maria da | |
| Author | Pinto, Eduardo Fonseca | |
| Access date | 2016-04-12T16:55:26Z | |
| Available date | 2016-04-12T16:55:26Z | |
| Document date | 2015 | |
| Citation | COUTO, Luzinei da Silva et al. Intranasal Vaccination with Leishmanial Antigens Protects Golden Hamsters (Mesocricetus auratus) Against Leishmania (Viannia) braziliensis Infection. Plos One, v. 9, n. 1, p. 1-7, Jan. 2015. | pt_BR |
| ISSN | 1932-6203 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13733 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Title | Intranasal Vaccination with Leishmanial Antigens Protects Golden Hamsters (Mesocricetus auratus) Against Leishmania (Viannia) braziliensis Infection | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pntd.0003439 | |
| Abstract | Background: Previous results have shown that oral and intranasal administration of particulate Leishmania (Leishmania) amazonensis antigens (LaAg) partially protects mice against L. amazonensis infection. However, vaccination studies on species of the subgenus Viannia, the main causative agent of cutaneous and mucosal leishmaniasis in the Americas, have been hampered by the lack of easy-to-handle bio-models that accurately mimic the human disease. Recently, we demonstrated that the golden hamster is an appropriate model for studying the immunopathogenesis of cutaneous leishmaniasis caused by L. (Viannia) braziliensis. Using the golden hamster model, our current study investigated whether the protective effect of intranasal immunisation with LaAg can be extended to L. braziliensis infection. Methodology/Principal Findings: Golden hamsters vaccinated with either two intranasal (IN) doses of LaAg (10 mg) or two intramuscular doses of LaAg (20 mg) were challenged 2 weeks post-vaccination with L. braziliensis. The results showed that
IN immunisation with LaAg significantly reduced lesion growth and parasitic load as well as serum IgG and IgG2 levels. At the experimental endpoint on day 114 post-infection, IN-immunised hamsters that were considered protected expressed IFN-c and IL10 mRNA levels that returned to uninfected skin levels. In contrast to the nasal route, intramuscular (IM)
immunisation failed to provide protection. Conclusions/Significance: These results demonstrate for the first time that the nasal route of immunisation can induce cross protection against L. braziliensis infection. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho (IBCCF). Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Leishmania (Viannia) braziliensis | pt_BR |
| Subject | Infection | pt_BR |
| Subject | Immunization | pt_BR |
| Subject | Mesocricetus | pt_BR |
| Subject | Intranasal Vaccination | pt_BR |
| DeCS | Mesocricetus | pt_BR |
| DeCS | Leishmania braziliensis | pt_BR |
| DeCS | Infecção | pt_BR |
| DeCS | Imunização | pt_BR |
| DeCS | Vacinação | pt_BR |
