| Author | Santos, Luciane Amorim | |
| Author | Gray, Rebecca R | |
| Author | Cunha, Joana Paixão Monteiro | |
| Author | Strazza, Evandra | |
| Author | Kashima, Simone G | |
| Author | Santos, Edson de Souza | |
| Author | Araújo, Thessika Hialla Almeida | |
| Author | Gonçalves, Marilda de Souza | |
| Author | Salemi, Marco | |
| Author | Alcantara, Luiz Carlos Júnior | |
| Access date | 2016-04-13T19:15:48Z | |
| Available date | 2016-04-13T19:15:48Z | |
| Document date | 2015 | |
| Citation | SANTOS, L. A. et al. Short communication: phylodynamics analysis of the human immunodeficiency virus type 1 envelope gene in mother and child pairs. AIDS Research Human Retroviruses, v. 31, n. 9, p. 913-920, 2015. | pt_BR |
| ISSN | 0889-2229 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13754 | |
| Language | eng | pt_BR |
| Publisher | Mary Ann Liebert | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | HIV | pt_BR |
| Subject in Portuguese | HIV-1 | pt_BR |
| Subject in Portuguese | Transmissão | pt_BR |
| Subject in Portuguese | Amamentação | pt_BR |
| Subject in Portuguese | Feminino | pt_BR |
| Subject in Portuguese | Criança | pt_BR |
| Title | Short communication: phylodynamics analysis of the human immunodeficiency virus type 1 envelope gene in mother and child pairs | pt_BR |
| Type | Article | pt_BR |
| Abstract | Characterizing the impact of HIV transmission routes on viral genetic diversity can improve the understanding of
the mechanisms of virus evolution and adaptation. HIV vertical transmission can occur in utero, during delivery,
or while breastfeeding. The present study investigated the phylodynamics of the HIV-1 env gene in mother-tochild
transmission by analyzing one chronically infected pair from Brazil and three acutely infected pairs from
Zambia, with three to five time points. Sequences from 25 clones from each sample were obtained and aligned
using Clustal X. ML trees were constructed in PhyML using the best evolutionary model. Bayesian analyses
testing the relaxed and strict molecular clock were performed using BEAST and a Bayesian Skyline Plot (BSP)
was construed. The genetic variability of previously described epitopes was investigated and compared between
each individual time point and between mother and child sequences. The relaxed molecular clock was the bestfitted
model for all datasets. The tree topologies did not show differentiation in the evolutionary dynamics of the
virus circulating in the mother from the viral population in the child. In the BSP, the effective population size was
more constant in time in the chronically infected patients while in the acute patients it was possible to detect
bottlenecks. The genetic variability within viral epitopes recognized by the human immune system was considerably
higher among the chronically infected pair in comparison with acutely infected pairs. These results
contribute to a better understanding of HIV-1 evolutionary dynamics in mother-to-child transmission. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratorio de Hematologia Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Florida College of Medicine. Department of Pathology, Immunology, and Laboratory Medicine. Gainesville, Florida | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratorio de Hematologia Genética e Biologia Computacional. Salvador, BA, Brasil / Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Universidade de São Paulo. Hemocentro de Ribeirão Preto. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratorio de Hematologia Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratorio de Hematologia Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratorio de Hematologia Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Florida College of Medicine. Department of Pathology, Immunology, and Laboratory Medicine. Gainesville, Florida | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratorio de Hematologia Genética e Biologia Computacional. Salvador, BA, Brasil | pt_BR |
| xmlui.metadata.dc.subject.ods | 05 Igualdade de gênero | |
