| Author | Barbosa, Alynne da Silva | |
| Author | Bastos, Otilio Machado Pereira | |
| Author | Uchôa, Claudia M. Antunes | |
| Author | Pissinatti, Alcides | |
| Author | Ferreira Filho, Paulo Ricardo | |
| Author | Dib, Laís Verdan | |
| Author | Azevedo, Eduarda Peixoto | |
| Author | Siqueira, Mayara Perlingeiro | |
| Author | Cardozo, Matheus Lessa | |
| Author | Amendoeira, Maria Regina Reis | |
| Access date | 2016-04-14T11:35:20Z | |
| Available date | 2016-04-14T11:35:20Z | |
| Document date | 2015 | |
| Citation | BARBOSA, Alynne da Silva; et al. Isolation and maintenance of Balantidium coli (Malmsteim, 1857) cultured from fecal samples of pigs and non-human primates. Veterinary Parasitology, v.210, n.3-4, p.240-245, 2015. | pt_BR |
| ISSN | 0304-4017 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13757 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Isolamento | pt_BR |
| Subject in Portuguese | Suínos | pt_BR |
| Subject in Portuguese | Primatas não-humanos | pt_BR |
| Subject in Portuguese | Balantidium | pt_BR |
| Title | Isolation and maintenance of Balantidium coli (Malmsteim, 1857) cultured from fecal samples of pigs and non-human primates | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vetpar.2015.03.030 | |
| Abstract | Balantidium coli is a protozoa that can determine dysentery in humans, pigs and non-human
primates having zoonotic potential. The lack of standardization in isolation and maintenance
hinders the development of research on its biology and epidemiology. This study is
aimed to standardize the isolation and maintenance of this parasite from animal feces, in
culture medium, Pavlova modified. From 2012 to 2014, 1905 fecal samples were collected
from captive animals of Rio de Janeiro. Were selected for isolation samples with a minimum
of 10 trophozoites and/or 30 cysts of B. coli, totaling 88 pigs, 26 Cynomolgus and 90 rhesus
macaques. In the presence of cysts, the sample was homogenized in saline solution, 500 L
was removed and inoculated into culture medium. The material that contained trophozoites
the inoculum was made from 240 L of fecal solution. All inoculate tubes with the
subcultures were kept at 36 ◦C, and sterile rice starch was always added to the medium.
The parasites isolate from pigs, 34%, and from Cynomolgus 38.4% were maintained in vitro
for a period of more than 24 months. These procedures proved to be adequate for isolation
and maintenance of B. coli from different animals, they were found to be inexpensive and
easy to perform. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Centro de Primatas do Rio de Janeiro. Guapimirm, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Criações de Animais de Laboratório (CECAL). Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxoplasmose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Balantidium coli | pt_BR |
| Subject | Isolation | pt_BR |
| Subject | Xenic cultivation | pt_BR |
| Subject | Pig | pt_BR |
| Subject | Non-human primates | pt_BR |
| Subject | Brazil | pt_BR |
| e-ISSN | 1873-2550 | |
