| Author | Ferreira, Alex Guerra | |
| Author | Leão, Robson Souza | |
| Author | Assef, Ana Paula D’Alincourt Carvalho | |
| Author | Silva, Érica Aparecida dos Santos RIbeiro da | |
| Author | Firmida, Monica de Cássia | |
| Author | Folescu, Tania Wrobel | |
| Author | Paixão, Vilma Almeida | |
| Author | Santana, Maria Angélica | |
| Author | Silva, Fernando Antonio de Abreu e | |
| Author | Barth, Afonso Luís | |
| Author | Marques, Elizabeth Andrade | |
| Access date | 2016-04-18T11:28:11Z | |
| Available date | 2016-04-18T11:28:11Z | |
| Document date | 2015 | |
| Citation | FERREIRA, Alex Guerra et al. Low-level resistance and clonal diversity of Pseudomonas aeruginosa among chronically colonized cystic fibrosis patients. APMIS, n. 123, p. 1061-1068, 2015. | pt_BR |
| ISSN | 0903-4641 | |
| URI | https://www.arca.fiocruz.br/handle/icict/13836 | |
| Language | eng | pt_BR |
| Publisher | Wiley | pt_BR |
| Rights | restricted access | |
| Title | Low-level resistance and clonal diversity of Pseudomonas aeruginosa among chronically colonized cystic fibrosis patients | pt_BR |
| Type | Article | |
| DOI | 10.1111/apm.12463 | |
| Abstract | A prospective study was conducted in Brazil to evaluate antimicrobial resistance patterns and molecular epidemiology of Pseudomonas aeruginosa isolates from cystic fibrosis (CF) patients with chronic lung infection. All isolates were obtained between May 2009 and June 2010 from 75 patients seen in four reference centers in Brazil: HCPA (20 patients) and HEOM (15 patients), located in southern and northeastern Brazil, respectively; IFF (20 patients) and HUPE (20 patients), both in southwestern Brazil. Antimicrobial susceptibility testing, PCR for detection of carpapenemases, and pulsed-field gel electrophoresis (PFGE) were performed in 274 isolates. A total of 224 PFGE types were identified and no clones were found circulating among the centers or within the same center. Despite the chronic infection, most patients were colonized by intermittent clones. Only three patients (4%) maintained the same clone during the study. The resistance rates were lower than 30% for the majority of antimicrobials tested in all centers and only 17% of isolates were multiresistant. Isolates (n = 54) with reduced susceptibility to imipenem and/or meropenem presented negative results for blaSPM-1, blaIMP 1, blaVIM, and blaKPCgenes. Our results indicate an unexpected low level of antimicrobial resistance and a high genotypic diversity among P. aeruginosa from Brazilian chronic CF patients. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Infecção Hospitalar. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Rio de Janeiro,. RJ, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Hospital Universitário Pedro Ernesto. Rio de Janeiro,. RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Fernandes Figueira. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Hospital Especializado Octavio Mangabeira. Serviço de Pneumologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Hospital Especializado Octavio Mangabeira. Serviço de Pneumologia. Salvador, BA, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Faculdade de Medicina. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio Grande do Sul. Hospital de Clínicas de Porto Alegre. Centro de Pesquisas Experimental. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Disciplina de Microbiologia e Imunologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Cystic fibrosis | pt_BR |
| Subject | Pseudomonas aeruginosa | pt_BR |
| Subject | Antimicrobial resistance | pt_BR |
| Subject | Molecular epidemiology | pt_BR |
| DeCS | Pseudomonas aeruginosa | pt_BR |
| DeCS | Epidemiologia Molecular | pt_BR |
| DeCS | Fibrose Cística | pt_BR |
| e-ISSN | 1600-0463 | |
| Embargo date | 2030-12-31 | |
