Author | Fraga, Deborah Bittencourt Mothé | |
Author | Pacheco, Luciano Vasconcellos | |
Author | Borja, Lairton Souza | |
Author | Tuy, Pétala Gardênia da Silva Estrela | |
Author | Bastos, Leila Andrade | |
Author | Solcà, Manuela da Silva | |
Author | Amorim, Leila Denise Alves Ferreira | |
Author | Veras, Patrícia Sampaio Tavares | |
Access date | 2016-04-19T18:07:54Z | |
Available date | 2016-04-19T18:07:54Z | |
Document date | 2016 | |
Citation | FRAGA, D. B. M. et al. The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs. PLOS Neglected Tropical Diseases, v. 10, n. 1, e0004333, 2016. | pt_BR |
ISSN | 1935-2727 | |
ISSN | 10.1371/journal.pntd.0004333 | |
URI | https://www.arca.fiocruz.br/handle/icict/13876 | |
Language | eng | pt_BR |
Publisher | Public Library of Science | pt_BR |
Rights | open access | pt_BR |
Subject in Portuguese | Leishmania | pt_BR |
Subject in Portuguese | Leishmaniose visceral | pt_BR |
Subject in Portuguese | Animal | pt_BR |
Subject in Portuguese | Cães | pt_BR |
Subject in Portuguese | Saúde pública | pt_BR |
Subject in Portuguese | Células | pt_BR |
Subject in Portuguese | Diagnóstico | pt_BR |
Title | The Rapid Test Based on Leishmania infantum Chimeric rK28 Protein Improves the Diagnosis of Canine Visceral Leishmaniasis by Reducing the Detection of False-Positive dogs | pt_BR |
Type | Article | pt_BR |
Abstract | Visceral Leishmaniasis (VL) has spread to many urban centers worldwide. Dogs are considered
the main reservoir of VL, because canine cases often precede the occurrence of
human cases. Detection and euthanasia of serologically positive dogs is one of the primary
VL control measures utilized in some countries, including Brazil. Using accurate diagnostic
tests can minimize one undesirable consequence of this measure, culling false-positive
dogs, and reduce the maintenance of false-negative dogs in endemic areas. In December
2011, the Brazilian Ministry of Health replaced the ELISA (EIE CVL) screening method and
Indirect Immunofluorescence Test (IFI CVL) confirmatory method with a new protocol using
the rapid DPP CVL screening test and EIE CVL confirmatory test. A study of diagnostic
accuracy of these two protocols was done by comparing their performance using serum
samples collected from a random sample of 780 dogs in an endemic area of VL. All samples
were evaluated by culture and real time PCR; 766 out of the 780 dogs were tested using the
previous protocol (IFI CVL + EIE CVL) and all 780 were tested using the current protocol
(DPP CVL + EIE CVL). Performances of both diagnostic protocols were evaluated using a
latent class variable as the gold standard. The current protocol had a higher specificity (0.98
vs. 0.95) and PPV (0.83 vs. 0.70) than the previous protocol, although sensitivity of these
two protocols was similar (0.73). When tested using sera from asymptomatic animals, the
current protocol had a much higher PPV (0.63 vs. 0.40) than the previous protocol (although
the sensitivity of either protocol was the same, 0.71). Considering a range of theoretical
CVL prevalences, the projected PPVs were higher for the current protocol than for the previous
protocol for each theoretical prevalence value. The findings presented herein show that the current protocol performed better than previous protocol primarily by reducing false-positive
results. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Universidade Federal da Bahia. Escola de Medicina Veterinária e Zootecnia. Departamento de Medicina Veterinária Preventiva e Produção Animal. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil | pt_BR |
Affilliation | Universidade Federal da Bahia. Instituto de Matemática. Departamento de Estatística. Salvador, BA, Brasil | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Patologia e Biointervenção. Salvador, BA, Brasil / Instituto de Ciência e Tecnologia de Doenças Tropicais. INCT-DT. Salvador, BA, Brasil | pt_BR |
xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |