| Author | Duarte, Ângelo | |
| Author | Queiroz, Artur Trancoso Lopo de | |
| Author | Tosta, Rafael | |
| Author | Carvalho, Augusto M | |
| Author | Barbosa, Carlos Henrique | |
| Author | Bellio, Maria | |
| Author | Oliveira, Camila Indiani de | |
| Author | Barral Netto, Manoel | |
| Access date | 2016-04-29T16:30:54Z | |
| Available date | 2016-04-29T16:30:54Z | |
| Document date | 2015 | |
| Citation | DUARTE. A. et al. Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation. PLoS One, v. 10, p. 0124786, 2015. | pt_BR |
| ISSN | 1932-6203 | |
| URI | https://www.arca.fiocruz.br/handle/icict/14037 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Subject in Portuguese | Leishmania braziliensis | pt_BR |
| Subject in Portuguese | Vacina contra Leishmaniose | pt_BR |
| Subject in Portuguese | CD8 | pt_BR |
| Subject in Portuguese | Epítopos | pt_BR |
| Subject in Portuguese | Validação social em pesquisa | pt_BR |
| Title | Prediction of CD8+ Epitopes in Leishmania braziliensis Proteins Using EPIBOT: In Silico Search and In Vivo Validation | pt_BR |
| Type | Article | pt_BR |
| Abstract | Leishmaniasis is caused by intracellular Leishmania parasites that induce a T-cell mediated
response associated with recognition of CD4+ and CD8+ T cell Line 1Lineepitopes. Identification
of CD8+ antigenic determinants is crucial for vaccine and therapy development.
Herein, we developed an open-source software dedicated to search and compile data obtained
from currently available on line prediction algorithms.
Methodology/Principal Findings
We developed a two-phase algorithm and implemented in an open source software called
EPIBOT, that consolidates the results obtained with single prediction algorithms, generating
a final output in which epitopes are ranked. EPIBOT was initially trained using a set of 831
known epitopes from 397 proteins from IEDB. We then screened 63 Leishmania braziliensis
vaccine candidates with the EPIBOT trained tool to search for CD8+ T cell epitopes. A
proof-of-concept experiment was conducted with the top eight CD8+ epitopes, elected by
EPIBOT. To do this, the elected peptides were synthesized and validated for their in vivo cytotoxicity.
Among the tested epitopes, three were able to induce lysis of pulsed-target cells.
Conclusion
Our results show that EPIBOT can successfully search across existing prediction tools, generating
a compiled list of candidate CD8+ epitopes. This software is fast and a simple search
engine that can be customized to search over different MHC alleles or HLA haplotypes. | pt_BR |
| Affilliation | Universidade Estadual de Feira de Santana. Departamento de Tecnologia. Feira de Santana, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil | pt_BR |
| Affilliation | Universidade Estadual de Feira de Santana. Departamento de Tecnologia. Feira de Santana, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Microbiologia Paulo de Góes. Centro de Ciências da Saúde. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Ba, Brasil / Instituto de Investigação em Imunologia. São Paulo, SP, Brasil | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
