| Author | Pitanga, Thassila Nogueira | |
| Author | Oliveira, Ricardo Riccio | |
| Author | Zanette, Dalila Lucíola | |
| Author | Guarda, Caroline Conceição da | |
| Author | Santiago, Rayra Pereira | |
| Author | Santana, Sanzio Silva | |
| Author | Nascimento, Valma M. L | |
| Author | Lima, Jonilson Berlink | |
| Author | Carvalho, Graziele Quintela | |
| Author | Maffili, Vitor Valério | |
| Author | Carvalho, Magda Oliveira Seixas | |
| Author | Alcantara, Luiz Carlos Júnior | |
| Author | Borges, Valéria de Matos | |
| Author | Goncalves, Marilda de Souza | |
| Access date | 2016-05-09T18:29:16Z | |
| Available date | 2016-05-09T18:29:16Z | |
| Document date | 2016 | |
| Citation | PITANGA, T. N. et al. Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell. Cytokine, v. 83, p. 75–84, 2016. | pt_BR |
| ISSN | 1043-4666 | |
| URI | https://www.arca.fiocruz.br/handle/icict/14136 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Title | Sickle red cells as danger signals on proinflammatory gene expression, leukotriene B4 and interleukin-1 beta production in peripheral blood mononuclear cell | pt_BR |
| Type | Article | pt_BR |
| DOI | dx.doi.org/10.1016/j.cyto.2016.03.016 | |
| Abstract | This study tested the hypothesis that sickle red blood cell (SS-RBC) induce Toll-like receptors (TLR) and
Nod-like receptor family, pyrin domain containing 3 (NLRP3)- inflammasome expression in peripheral
blood mononuclear cells (PBMC). TLR and NLRP3 inflammasome could contribute to the maintenance
of the inflammatory status in sickle cell anemia (SCA) patients, since SS-RBC act as danger signals activating
these pathways. In this study, first, we evaluated TLR (2, 4, 5 and 9), NLRP3, Caspase-1, interleukin (IL)-
1b and IL-18 expression in PBMC freshly isolated from SCA patients (SS-PBMC) in comparison with PBMC
from healthy individuals (AA-PBMC). In the second moment, we investigated whether SS-RBC could
interfere with the expression of these molecules in PBMC from healthy donor, in the absence or presence
of hydroxyurea (HU) in vitro. TLRs and NLRP3 inflammasome expression were investigated by qPCR. IL-
1b, Leukotriene-B4 (LTB4) and nitrite production were measured in PBMC (from healthy donor) culture
supernatants. TLR2, TLR4, TLR5, NLRP3 and IL-1b were highly expressed in SS-PBMC when compared to
AA-PBMC. Additionally, SS-RBC induced TLR9, NLRP3, Caspase-1, IL-1b and IL-18 expression and induced
IL-1b, LTB4 and nitrite production in PBMC cultures. HU did not prevent TLR and NLRP3 inflammasome
expression, but increased TLR2 and IL-18 expression and reduced nitrite production. In conclusion, our
data suggest that TLR and inflammasome complexes may be key inducers of inflammation in SCA
patients, probably through SS-RBC; also, HU does not prevent NLRP3 inflammasome- and
TLR-dependent inflammation, indicating the need to develop new therapeutic strategies to SCA patients
that act with different mechanisms of those observed for HU. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação de Hematologia e Hemoterapia da Bahia. HEMOBA. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Hospital Universitário Professor Edgard Santos. HUPES. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmácia, Salvador, BA, Brasil | pt_BR |
| Subject | Sickle cell anemia | pt_BR |
| Subject | Sickle red cell | pt_BR |
| Subject | IL-1B | pt_BR |
| Subject | Leukotriene B4 | pt_BR |
| Subject | NLRP3 inflammasome | pt_BR |
| Subject | Toll-like receptors | pt_BR |
