| Author | Alberto, Anael Viana Pinto | |
| Author | Faria, Robson Xavier | |
| Author | Menezes, João Ricardo Lacerda de | |
| Author | Surrage, Andrea | |
| Author | Rocha, Natasha Cristina da | |
| Author | Ferreira, Leonardo Gomes Braga | |
| Author | Frutuoso, Valber da Silva | |
| Author | Martins, Marco Aurélio | |
| Author | Nunes, Luiz Anastácio | |
| Access date | 2016-05-11T13:05:03Z | |
| Available date | 2016-05-11T13:05:03Z | |
| Document date | 2016 | |
| Citation | ALBERTO, Anael Viana Pinto; et al. Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation. Plos One, v.11, n.1, e0145392, 17p, Jan. 2016. | pt_BR |
| ISSN | 1932-6203 | |
| URI | https://www.arca.fiocruz.br/handle/icict/14235 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Eosinófilos | pt_BR |
| Subject in Portuguese | Inflamação alérgica | pt_BR |
| Subject in Portuguese | Eosinófilos de rato | pt_BR |
| Subject in Portuguese | P2 Receptores | pt_BR |
| Title | Role of P2 Receptors as Modulators of Rat Eosinophil Recruitment in Allergic Inflammation | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal. pone.0145392 | |
| Abstract | ATP and other nucleotides are released from cells through regulated pathways or following
the loss of plasma membrane integrity. Once outside the cell, these compounds can activate
P2 receptors: P2X ionotropic receptors and G protein-coupled P2Y receptors. Eosinophils
represent major effector cells in the allergic inflammatory response and they are, in
fact, associated with several physiological and pathological processes. Here we investigate
the expression of P2 receptors and roles of those receptors in murine eosinophils. In this
context, our first step was to investigate the expression and functionality of the P2X receptors
by patch clamping, our results showed a potency ranking order of ATP>ATPγS>
2meSATP> ADP> αβmeATP> βγmeATP>BzATP> UTP> UDP>cAMP. This data suggest
the presence of P2X1, P2X2 and P2X7. Next we evaluate by microfluorimetry the expression
of P2Y receptors, our results based in the ranking order of potency (UTP>ATPγS>
ATP > UDP> ADP >2meSATP > αβmeATP) suggests the presence of P2Y2, P2Y4, P2Y6
and P2Y11. Moreover, we confirmed our findings by immunofluorescence assays. We also
did chemotaxis assays to verify whether nucleotides could induce migration. After 1 or 2
hours of incubation, ATP increased migration of eosinophils, as well as ATPγS, a less
hydrolysable analogue of ATP, while suramin a P2 blocker abolished migration. In keeping
with this idea, we tested whether these receptors are implicated in the migration of eosinophils
to an inflammation site in vivo, using a model of rat allergic pleurisy. In fact, migration
of eosinophils has increased when ATP or ATPγS were applied in the pleural cavity, and
once more suramin blocked this effect. We have demonstrated that rat eosinophils express
P2X and P2Y receptors. In addition, the activation of P2 receptors can increase migration of
eosinophils in vitro and in vivo, an effect blocked by suramin. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Ciências Biomédicas. Laboratório de Neuroanatomia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inflamação. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Comunicação Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | P2 Receptors | pt_BR |
| Subject | Allergic Inflammation | pt_BR |
| Subject | Rat eosinophils | pt_BR |
