| Author | Nasaré, Alex M. | |
| Author | Tedesco, Roberto C. | |
| Author | Cristovam, Priscila C. | |
| Author | Cenedese, Marcos A. | |
| Author | Galisteo Jr., Andrés J. | |
| Author | Andrade Jr., Heitor F. | |
| Author | Gomes, José Álvaro P. | |
| Author | Guimarães, Érik V. | |
| Author | Barbosa, Helene S. | |
| Author | Alonso, Luis G. | |
| Access date | 2016-05-19T16:11:15Z | |
| Available date | 2016-05-19T16:11:15Z | |
| Document date | 2015 | |
| Citation | NASARÉ, Alex M. et al. Toxoplasma Gondii Infection of Chicken Embryos Causes Retinal Changes and Modulates HSP90B1 Gene Expression: A Promising Ocular Toxoplasmosis Model. Eur J Microbiol Immunol (Bp), v.5, n.4, p.316-20, Nov. 2015. | pt_BR |
| ISSN | 2062-8633 | |
| URI | https://www.arca.fiocruz.br/handle/icict/14397 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Desenvolvimento embrionário | pt_BR |
| Subject in Portuguese | Modelos experimentais | pt_BR |
| Subject in Portuguese | Toxoplasmose ocular | pt_BR |
| Subject in Portuguese | Infecções oculares parasitárias | pt_BR |
| Subject in Portuguese | Retina | pt_BR |
| Title | Toxoplasma gondii infection of chicken embryos causes retinal changes and modulates HSP90B1 gene expression: a promising ocular toxoplasmosis model | pt_BR |
| Type | Article | |
| DOI | 10.1556/1886.2015.00024 | |
| Abstract | HSP90B1 is a gene that codifies heat shock protein 108 (HSP108) that belongs to a group of proteins induced under stress situation, and it has close relation with the nervous system, especially in the retina. Toxoplasma gondii causes ocular toxoplasmosis that has been associated with a late manifestation of the congenital toxoplasmosis although experimental models show that morphological alterations are already present during embryological development. Here, we used 18 eyes of Gallus domesticus embryos in 7th and 20th embryonic days to establish a model of congenital ocular toxoplasmosis, experimentally infected in its fifth day correlating with HSP90B1 gene expression. Embryos' eyes were histologically evaluated, and gene expression was performed by real-time polymerase chain reaction (PCR). Our data showed parasite present in the choroid, unusual migration of retinal pigment epithelium, and chorioretinal scars, and a tendency to a lower expression of the HSP90B1 gene upon experimental infection. This is a promising model to better understand T. gondii etiopathogeny. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Instituto de Medicina Tropical de São Paulo. Departamento de Oftalmologia. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Disciplina de Nefrologia. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Protozoologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Protozoologia. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Instituto de Medicina Tropical de São Paulo. Departamento de Oftalmologia. Botucatu, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo. Departamento de Genética e Morfologia. Botucatu, SP, Brasil. | pt_BR |
| Subject | Embryo development | pt_BR |
| Subject | Experimental models | pt_BR |
| Subject | Ocular toxoplasmosis | pt_BR |
| Subject | Parasitic ocular infections | pt_BR |
| Subject | Regulation of gene expression in developmen | pt_BR |
| Subject | Retina | pt_BR |
