| Author | Albuquerque, Juliana Barreto de | |
| Author | Santos, Danielle Silva dos | |
| Author | Pérez, Ana Rosa | |
| Author | Berbert, Luiz Ricardo | |
| Author | van Vliet, Eliane de Santana | |
| Author | Oliveira, Désio Aurélio Farias de | |
| Author | Moreira, Otacilio C. | |
| Author | Roggero, Eduardo | |
| Author | Pinto, Carla Eponina de Carvalho | |
| Author | Jurberg, José | |
| Author | Almeida, Vinicius Cotta de | |
| Author | Botasso, Oscar | |
| Author | Savino, Wilson | |
| Author | Meis, Juliana de | |
| Access date | 2016-05-19T17:15:35Z | |
| Available date | 2016-05-19T17:15:35Z | |
| Document date | 2015 | |
| Citation | ALBUQUERQUE, Juliana Barreto de; et al. Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection?. PLoS Negl Trop Dis., v.9, n.6, 21p, June 2015. | pt_BR |
| ISSN | 1935-2735 | |
| URI | https://www.arca.fiocruz.br/handle/icict/14400 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Transmissão oral | pt_BR |
| Subject in Portuguese | Doença de Chagas | pt_BR |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Surto | pt_BR |
| Subject in Portuguese | Relação parasita-hospedeiro | pt_BR |
| Subject in Portuguese | Ratos | pt_BR |
| Title | Trypanosoma cruzi Infection through the Oral Route Promotes a Severe Infection in Mice: New Disease Form from an Old Infection? | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pntd.0003849 | |
| Abstract | Oral transmission of Chagas disease has been documented in Latin American countries.
Nevertheless, significant studies on the pathophysiology of this form of infection are largely
lacking. The few studies investigating oral route infection disregard that inoculation in the
oral cavity (Oral infection, OI) or by gavage (Gastrointestinal infection, GI) represent different
infection routes, yet both show clear-cut parasitemia and heart parasitism during the
acute infection. Herein, BALB/c mice were subjected to acute OI or GI infection using 5x104
culture-derived Trypanosoma cruzi trypomastigotes. OI mice displayed higher parasitemia
and mortality rates than their GI counterparts. Heart histopathology showed larger areas of
infiltration in the GI mice, whereas liver lesions were more severe in the OI animals, accompanied
by higher Alanine Transaminase and Aspartate Transaminase serum contents. A
differential cytokine pattern was also observed because OI mice presented higher proinflammatory
cytokine (IFN-γ, TNF) serum levels than GI animals. Real-time PCR confirmed
a higher TNF, IFN-γ, as well as IL-10 expression in the cardiac tissue from the OI group
compared with GI. Conversely, TGF-β and IL-17 serum levels were greater in the GI animals.
Immunolabeling revealed macrophages as the main tissue source of TNF in infected
mice. The high mortality rate observed in the OI mice paralleled the TNF serum rise, with its
inhibition by an anti-TNF treatment. Moreover, differences in susceptibility between GI versus
OI mice were more clearly related to the host response than to the effect of gastric pH
on parasites, since infection in magnesium hydroxide-treated mice showed similar results.
Overall, the present study provides conclusive evidence that the initial site of parasite
entrance critically affects host immune response and disease outcome. In light of the occurrence
of oral Chagas disease outbreaks, our results raise important implications in terms of
the current view of the natural disease course and host-parasite relationship. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | National University of Rosario. Faculty of Medical Science. Immunology Institute. Rosario, Argentina. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, Rj, Brasil. | pt_BR |
| Affilliation | National University of Rosario. Faculty of Medical Science. Immunology Institute. Rosario, Argentina. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Departamento de Imunobiologia. Laboratório de Patologia Experimental. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Nacional e Internacional de Referência em Taxonomia de Triatomíneos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | National University of Rosario. Faculty of Medical Science. Immunology Institute. Rosario, Argentina. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Mice | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Oral transmission | pt_BR |
| Subject | Oral Chagas disease outbreaks | pt_BR |
| Subject | Host-parasite relationship | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
