| Author | Siqueira, Liliane Maria Vidal | pt_BR |
| Author | Gomes, Luciana Inácia | pt_BR |
| Author | Oliveira, Edward José de | pt_BR |
| Author | Oliveira, Eduardo Ribeiro de | pt_BR |
| Author | Oliveira, Áureo Almeida de | pt_BR |
| Author | Enk, Martin Johannes | pt_BR |
| Author | Carneiro, Nídia Figueiredo | pt_BR |
| Author | Rabello, Ana Lucia Teles | pt_BR |
| Author | Coelho, Paulo Marcos Zech | pt_BR |
| Access date | 2016-06-16T17:21:47Z | pt_BR |
| Access date | 2016-07-08T02:09:53Z | |
| Available date | 2016-06-16T17:21:47Z | pt_BR |
| Available date | 2016-07-08T02:09:53Z | |
| Document date | 2015 | |
| Citation | SIQUEIRA, Liliane Maria Vidal et al. Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 110, n. 2, p. 209-214, 2015. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14485 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz | pt_BR |
| Rights | open access | pt_BR |
| Title | Evaluation of parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni in a low transmission area | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1590/0074-02760140375 | pt_BR |
| Abstract | This study evaluated parasitological and molecular techniques for the diagnosis and assessment of cure of schistosomiasis mansoni. A population-based study was performed in 201 inhabitants from a low transmission locality named Pedra Preta, municipality of Montes Claros, state of Minas Gerais, Brazil. Four stool samples were analysed using two techniques, the Kato-Katz® (KK) technique (18 slides) and the TF-Test®, to establish the infection rate. The positivity rate of 18 KK slides of four stool samples was 28.9% (58/201) and the combined parasitological techniques (KK+TF-Test®) produced a 35.8% positivity rate (72/201). Furthermore, a polymerase chain reaction (PCR)-ELISA assay produced a positivity rate of 23.4% (47/201) using the first sample. All 72 patients with positive parasitological exams were treated with a single dose of Praziquantel® and these patients were followed-up 30, 90 and 180 days after treatment to establish the cure rate. Cure rates obtained by the analysis of 12 KK slides were 100%, 100% and 98.4% at 30, 90 and 180 days after treatment, respectively. PCR-ELISA revealed cure rates of 98.5%, 95.5% and 96.5%, respectively. The diagnostic and assessment of cure for schistosomiasis may require an increased number of KK slides or a test with higher sensitivity, such as PCR-ELISA, in situations of very low parasite load, such as after therapeutic interventions. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Instituto Evandro Chagas. Ananindeua, PA, Brasil. | pt_BR |
| Affilliation | Prefeitura de Montes Claros. Centro de Controle de Zoonoses. Montes Claros, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Pesquisas Clínicas. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Schistosomiasis | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Low transmission area | pt_BR |
| Subject | Control | pt_BR |
