| Author | Degrave, Wim | |
| Author | Fragoso, Stenio P. | |
| Author | Britto, Constança | |
| Author | van Heuverswyn, Hugo | |
| Author | Kidane, Getachew Z. | |
| Author | Cardoso, Maria A. B. | |
| Author | Mueller, Rita U. | |
| Author | Simpson, Larry | |
| Author | Morel, Carlos Medicis | |
| Access date | 2016-06-29T12:39:45Z | pt_BR |
| Access date | 2016-07-07T11:40:02Z | |
| Available date | 2016-06-29T12:39:45Z | pt_BR |
| Available date | 2016-07-07T11:40:02Z | |
| Document date | 1988 | |
| Citation | DEGRAVE, Wim; et al. Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi. Molecular and Biochemical Parasitology, v.27, p.63-70 , 1988. | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14598 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Title | Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi | pt_BR |
| Type | Article | |
| DOI | 10.1016/0166-6851(88)90025-4 | pt_BR |
| Abstract | The sequences of two minicircles from the kinetoplast DNA of the CL strain and one of the Y strain of Trypanosoma cruzi are
reported. These 1.4 kb molecules have a peculiar sequence organization, the most distinctive feature being the occurrence of a
120 bp sequence repeated four times, located at 0, 90, 180 and 270 degrees along each circle. We have termed these conserved
regions in this species 'minirepeats'. Minirepeats have a 3-fold higher concentration of cytosine residues in comparison with the
variable regions and contain the universal 12-mer motif GGGGTTGGTGTA present in all sequenced minicircles and which was
shown to be involved in DNA replication. A consensus sequence of T. cruzi minirepeats was determined using the 20 minirepeats
present in five known T. cruzi minicircle sequences. This consensus sequence contains regions which have been remarkably well
preserved in strains which show great biological diversity. In addition a low level of intraminicircle sequence similarity was also
observed within the variable region, but this similarity did not extend between strains. The abundance of conserved minirepeat
sequences containing invariant restriction sites in T. cruzi cells may prove valuable for the development of new direct diagnostic
methods for Chagas' disease based on DNA probe technology. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | University of California. Biology and Molecular Biology Institute. Los Alngeles, CA, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Kinetoplast DNA sequence | pt_BR |
| Subject | Minicircle DNA | pt_BR |
| Subject | DNA sequence | pt_BR |
| DeCS | Trypanosoma cruzi | pt_BR |
| DeCS | Sequência de Bases | pt_BR |
| DeCS | DNA de Cinetoplasto | pt_BR |
| e-ISSN | 1872-9428 | pt_BR |
