Please use this identifier to cite or link to this item:
https://www.arca.fiocruz.br/handle/icict/14603
Type
ArticleCopyright
Open access
Collections
- IOC - Artigos de Periódicos [12491]
- MG - IRR - Artigos de Periódicos [4049]
Metadata
Show full item record
CHANGES IN THE ISOENZYME AND KINETOPLAST DNA PATTERNS OF TRYPANOSOMA CRUZI STRAINS INDUCED BY MAINTENANCE IN MICE
Author
Affilliation
Univeridade Federal de Minas Gerais. Departamento de Parasitologia e de Bioquimica. Belo Horizonte, MG, Brasil.
Univeridade Federal de Minas Gerais. Departamento de Parasitologia e de Bioquimica. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquimica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Univeridade Federal de Minas Gerais. Departamento de Parasitologia e de Bioquimica. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquimica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Univeridade Federal de Minas Gerais. Departamento de Parasitologia e de Bioquimica. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquimica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Univeridade Federal de Minas Gerais. Departamento de Parasitologia e de Bioquimica. Belo Horizonte, MG, Brasil.
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquimica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil.
Abstract
Culture forms of thirteen Trypanosoma cruzi strains from 4 zymodemes and 9 schizodemes were inoculated
and kept by successive passages in C3H mice. The strains were initially from the following zymodemes: 3
from A, 3 from B, 4 from C and 2 from D and 1 from AB mixed zymodemes. After approximately 18
months maintenance the parasites were isolated by hemoculture and again typed according to their
isoenzyme and kinetoplast DNA patterns. The zymodeme A strains kept their initial patterns; from the 3
zymodeme B strains, two kept the initial patterns and one changed to zymodeme A; from the 4 zymodeme
C, two kept the initial pattern and two changed to zymodeme B; from the 2 zymodeme D strains, one kept
the initial pattern and one changed to zymodeme A. The strain from AB mixed zymodeme was reduced to
zymodeme A. The zymodeme changes were accompanied by schizodeme changes. Although not
simultaneously, in one 7". cruzi strain the parasitemia change was followed by zymodeme and schizodeme
changes. The results showed that prolonged maintenance of T. cruzi in mice by successive passages alters
the isoenzyme and k-DNA patterns of some strains and that these alterations tend to move towards
zymodeme A, suggesting a selective effect of mice over these T. cruzi populations.
Share