| Author | Castro, Daniele P. | |
| Author | Moraes, Caroline S. | |
| Author | Gonzalez, Marcelo S. | |
| Author | Ratcliffe, Norman A. | |
| Author | Azambuja, Patrícia | |
| Author | Garcia, Eloi S. | |
| Access date | 2016-06-23T19:44:42Z | pt_BR |
| Access date | 2016-07-07T12:00:48Z | |
| Available date | 2016-06-23T19:44:42Z | pt_BR |
| Available date | 2016-07-07T12:00:48Z | |
| Document date | 2012 | |
| Citation | CASTRO, Daniele P. et al. Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development. Plos One, v.7, n.5, e36591. 8p, May 2012. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14649 | |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Microbiota | pt_BR |
| Subject in Portuguese | Intestino | pt_BR |
| Subject in Portuguese | Respostas imunes | pt_BR |
| Subject in Portuguese | Parasitos | pt_BR |
| Title | Trypanosoma cruzi Immune Response Modulation Decreases Microbiota in Rhodnius prolixus Gut and Is Crucial for Parasite Survival and Development | pt_BR |
| Type | Article | |
| DOI | 10.1371/journal.pone.0036591 | pt_BR |
| Abstract | Trypanosoma cruzi in order to complete its development in the digestive tract of Rhodnius prolixus needs to overcome the
immune reactions and microbiota trypanolytic activity of the gut. We demonstrate that in R. prolixus following infection
with epimastigotes of Trypanosoma cruzi clone Dm28c and, in comparison with uninfected control insects, the midgut
contained (i) fewer bacteria, (ii) higher parasite numbers, and (iii) reduced nitrite and nitrate production and increased
phenoloxidase and antibacterial activities. In addition, in insects pre-treated with antibiotic and then infected with Dm28c,
there were also reduced bacteria numbers and a higher parasite load compared with insects solely infected with parasites.
Furthermore, and in contrast to insects infected with Dm28c, infection with T. cruzi Y strain resulted in a slight decreased
numbers of gut bacteria but not sufficient to mediate a successful parasite infection. We conclude that infection of R.
prolixus with the T. cruzi Dm28c clone modifies the host gut immune responses to decrease the microbiota population and
these changes are crucial for the parasite development in the insect gut. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense (UFF). Instituto de Biologia. Departamento de Biologia Geral. Laboratório de Biologia de Insetos. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM). Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Bioquímica e Fisiologia de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Entomologia Molecular (INCT-EM). Departamento de Entomologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | Rhodnius prolixus | pt_BR |
| Subject | Microbiota | pt_BR |
| Subject | Gut immune responses | pt_BR |
| Subject | Parasite infection | pt_BR |
