| Author | Nogueira, Alanderson R. | |
| Author | Leve, Fernanda | |
| Author | Morgado-Diaz, José | |
| Author | Tedesco, Roberto Carlos | |
| Author | Pereira, Mirian Claudia S. | |
| Access date | 2016-07-05T12:54:49Z | pt_BR |
| Access date | 2016-07-08T12:21:56Z | |
| Available date | 2016-07-05T12:54:49Z | pt_BR |
| Available date | 2016-07-08T12:21:56Z | |
| Document date | 2016 | |
| Citation | NOGUEIRA, Alanderson R. et al. Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells. Parasitology, v.143, n.05, p.568-575, Apr. 2016. | pt_BR |
| ISSN | 0031-1820 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14768 | |
| Language | eng | pt_BR |
| Publisher | Cambridge University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Células epiteliais do pigmento da retina | pt_BR |
| Subject in Portuguese | Toxoplasma | pt_BR |
| Subject in Portuguese | Complexo juncional | pt_BR |
| Title | Effect of Toxoplasma gondii infection on the junctional complex of retinal pigment epithelial cells | pt_BR |
| Type | Article | |
| DOI | 10.1017/S0031182015001973 | pt_BR |
| Abstract | Ocular toxoplasmosis is the most frequent cause of uveitis, leading to partial or total loss of vision, with the retina the main affected structure. The cells of the retinal pigment epithelium (RPE) play an important role in the physiology of the retina and formation of the blood–retinal barrier. Several pathogens induce barrier dysfunction by altering tight junction (TJ) integrity. Here, we analysed the effect of infection by Toxoplasma gondii on TJ integrity in ARPE-19 cells. Loss of TJ integrity was demonstrated in T. gondii-infected ARPE-19 cells, causing increase in paracellular permeability and disturbance of the barrier function of the RPE. Confocal microscopy also revealed alteration in the TJ protein occludin induced by T. gondii infection. Disruption of junctional complex was also evidenced by scanning and transmission electron microscopy. Cell–cell contact loss was noticed in the early stages of infection by T. gondii with the visualization of small to moderate intercellular spaces. Large gaps were mostly observed with the progression of the infection. Thus, our data suggest that the alterations induced by T. gondii in the structural organization of the RPE may contribute to retinal injury evidenced by ocular toxoplasmosis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Coordenação de Pesquisa. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Nacional de Câncer. Coordenação de Pesquisa. Divisão de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Estrutural. Rio de Janeiro, RJ, Brasil / Universidade Federal de São Paulo. Disciplina de Anatomia Descritiva e Topográfica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultraestrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Toxoplasma gondii | pt_BR |
| Subject | Retinal pigment epithelial cells | pt_BR |
| Subject | Junctional complex | pt_BR |
| e-ISSN | 1469-8161 | pt_BR |
