| Author | Castro, Cássia | |
| Author | Menna-Barreto, Rubem Figueiredo Sadok | |
| Author | Fernandes, Nilma de Souza | |
| Author | Vahia, Leonardo Saboia | |
| Author | Lopes, Geovane Dias | |
| Author | Britto, Constança | |
| Author | Cuervo, Patricia | |
| Author | Jesus, José Batista de | |
| Access date | 2016-07-05T15:15:30Z | pt_BR |
| Access date | 2016-07-08T12:21:59Z | |
| Available date | 2016-07-05T15:15:30Z | pt_BR |
| Available date | 2016-07-08T12:21:59Z | |
| Document date | 2016 | |
| Citation | CASTRO, Cássia; et al. Iron-modulated pseudocyst formation in Tritrichomonas foetus. Parasitology, v.143, n.08, p.1034-1043, July 2016. | pt_BR |
| ISSN | 0031-1820 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14778 | |
| Language | eng | pt_BR |
| Publisher | Cambridge University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Transformação morfológica | pt_BR |
| Title | Iron-modulated pseudocyst formation in Tritrichomonas foetus | pt_BR |
| Type | Article | |
| DOI | 10.1017/S0031182016000573 | pt_BR |
| Abstract | Iron is an essential element for the survival of trichomonads during host–parasite interaction. The availability of this metal modulates several metabolic pathways of the parasites and regulates the expression of virulence factors such as adhesins and proteolytic enzymes. In this study, we investigated the effect of iron depletion on the morphology and life cycle of Tritrichomonas foetus. Scanning and transmission electron microscopy analyses revealed that depletion of iron from the culture medium (named TYM-DIP inducer medium) induces morphological transformation of typical pear-shaped trophozoites into spherical and non-motile pseudocysts. Remarkably, inoculation of pseudocysts into an iron-rich medium (standard TYM medium), or addition of FeSO4 to a TYM-DIP inducer medium reverted the morphological transformation process and typical trophozoites were recovered. These results show that pseudocysts are viable forms of the parasite and highlight the role of iron as a modulator of the parasite phenotype. Although iron is required for the survival of T. foetus, iron depletion does not cause a cellular collapse of pseudocysts, but instead induces phenotypic alterations, probably in order to allow the parasite to survive conditions of nutritional stress. Together, these findings support previous studies that suggest pseudocysts are a resistance form in the life cycle of T. foetus and enable new approaches to understanding the multifactorial role of iron in the cell biology of this protozoan parasite. | pt_BR |
| Affilliation | Universidade Federal de São João Del Rei. Departamento de Medicina. São João del Rei, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São João Del Rei. Departamento de Medicina. São João del Rei, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro. RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro. RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São João Del Rei. Departamento de Medicina. São João del Rei, MG, Brasil. | pt_BR |
| Subject | Tritrichomonas foetus | pt_BR |
| Subject | Pseudocyst | pt_BR |
| Subject | Iron | pt_BR |
| Subject | Morphological transformation | pt_BR |
| Subject | Trophozoites | pt_BR |
| DeCS | Trofozoítos | pt_BR |
| DeCS | Tritrichomonas foetus | pt_BR |
| DeCS | Pseudocisto Pancreático | pt_BR |
| DeCS | Ferro | pt_BR |
| e-ISSN | 1469-8161 | pt_BR |
