| Author | Pinto, Thiago Gomes de Toledo | |
| Author | Ferreira, Anna Beatriz Robottom | |
| Author | Alves, Marcelo Ribeiro | |
| Author | Rodrigues, Luciana Silva | |
| Author | Silva, Leonardo Ribeiro Batista | |
| Author | Silva, Bruno Jorge de Andrade | |
| Author | Lemes, Robertha Mariana Rodrigues | |
| Author | Martinez, Alejandra Nóbrega | |
| Author | Sandoval, Felipe Galvan | |
| Author | Alvarado-Arnez, Lucia Elena | |
| Author | Rosa, Patrícia Sammarco | |
| Author | Shannon, Edward Joseph | |
| Author | Pessolani, Maria Cristina Vidal | |
| Author | Pinheiro, Roberta Olmo | |
| Author | Antunes, Sérgio Luís Gomes | |
| Author | Sarno, Euzenir Nunes | |
| Author | Lara, Flávio Alves | |
| Author | Williams, Diana Lynn | |
| Author | Moraes, Milton Ozório | |
| Access date | 2016-07-05T16:50:33Z | pt_BR |
| Access date | 2016-07-08T12:22:01Z | |
| Available date | 2016-07-05T16:50:33Z | pt_BR |
| Available date | 2016-07-08T12:22:01Z | |
| Document date | 2016 | |
| Citation | PINTO, Thiago Gomes de Toledo; et al. STING-Dependent 2′-5′ Oligoadenylate Synthetase–Like Production Is Required for Intracellular Mycobacterium leprae Survival. The Journal od Infectious Diseases, v.214, n.2, p.311-20, July 2016. | pt_BR |
| ISSN | 1537-6613 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14782 | |
| Language | eng | pt_BR |
| Publisher | Oxford University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | Mycobacterium leprae | pt_BR |
| Subject in Portuguese | Sensor de DNA citoplasmático | pt_BR |
| Subject in Portuguese | Autofagia | pt_BR |
| Subject in Portuguese | Interferon Tipo I | pt_BR |
| Title | STING-Dependent 2′-5′ Oligoadenylate Synthetase–Like Production Is Required for Intracellular Mycobacterium leprae Survival | pt_BR |
| Type | Article | |
| DOI | 10.1093/infdis/jiw144 | pt_BR |
| Abstract | Cytosolic detection of nucleic acids elicits a type I interferon (IFN) response and plays a critical role in host defense against intracellular pathogens. Herein, a global gene expression profile of Mycobacterium leprae-infected primary human Schwann cells identified the genes differentially expressed in the type I IFN pathway. Among them, the gene encoding 2'-5' oligoadenylate synthetase-like (OASL) underwent the greatest upregulation and was also shown to be upregulated in M. leprae-infected human macrophage cell lineages, primary monocytes, and skin lesion specimens from patients with a disseminated form of leprosy. OASL knock down was associated with decreased viability of M. leprae that was concomitant with upregulation of either antimicrobial peptide expression or autophagy levels. Downregulation of MCP-1/CCL2 release was also observed during OASL knock down. M. leprae-mediated OASL expression was dependent on cytosolic DNA sensing mediated by stimulator of IFN genes signaling. The addition of M. leprae DNA enhanced nonpathogenic Mycobacterium bovis bacillus Calmette-Guerin intracellular survival, downregulated antimicrobial peptide expression, and increased MCP-1/CCL2 secretion. Thus, our data uncover a promycobacterial role for OASL during M. leprae infection that directs the host immune response toward a niche that permits survival of the pathogen. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto de Pesquisa Evandro Chagas. Laboratório de Pesquisa em Farmacogenética. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Pavilhão de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Pavilhão de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | National Hansen’s Disease Programs. Bureau of Primary Health Care. Health Resources and Services Administration. Baton Rouge, USA / Louisiana State University. School of Veterinary Medicine. Laboratory Research Branch. Baton Rouge, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Instituto Lauro de Souza Lima. Bauru, SP, Brasil. | pt_BR |
| Affilliation | National Hansen’s Disease Programs. Bureau of Primary Health Care. Health Resources and Services Administration. Baton Rouge, USA / Louisiana State University. School of Veterinary Medicine. Laboratory Research Branch. Baton Rouge, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Pavilhão de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Pavilhão de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | National Hansen’s Disease Programs. Bureau of Primary Health Care. Health Resources and Services Administration. Baton Rouge, USA / Louisiana State University. School of Veterinary Medicine. Laboratory Research Branch. Baton Rouge, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Leprosy | pt_BR |
| Subject | type I IFN | pt_BR |
| Subject | OASL | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | cytoplasmic DNA sensing | pt_BR |
| Subject | Autophagy | pt_BR |
| e-ISSN | 1537-6613 | pt_BR |
