| Author | Baptista, Abrahão Fontes | pt_BR |
| Author | Gomes, Joyce Rios de Souza | pt_BR |
| Author | Oliveira, Júlia Teixeria | pt_BR |
| Author | Santos, Soraia Moreira Garzedim | pt_BR |
| Author | Santos, Marcos André Vannier dos | pt_BR |
| Author | Martinez, Ana Maria Blanco | pt_BR |
| Access date | 2016-07-04T17:54:15Z | pt_BR |
| Access date | 2016-07-14T19:07:29Z | |
| Available date | 2016-07-04T17:54:15Z | pt_BR |
| Available date | 2016-07-14T19:07:29Z | |
| Document date | 2007 | |
| Citation | BAPTISTA, A. F. et al. A new approach to assess function after sciatic nerve lesion in the mouse-Adaptation of the sciatic static index. Journal of Neuroscience Methods, v. 161, p. 259–264, 2007. | pt_BR |
| ISSN | 0165-0270 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14912 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | pt_BR |
| Title | A new approach to assess function after sciatic nerve lesion in the mouse—Adaptation of the sciatic static index | pt_BR |
| Type | Article | pt_BR |
| Abstract | Among the numerous ways of assessing regeneration after peripheral nerve lesions, the analysis of gait is one of the most important, because it
shows the recovery of function, which is the ultimate goal of the repair machinery. The sciatic function index was introduced as a method to assess
reinnervation after an experimental sciatic nerve lesion, and was adapted to the mouse model. The sciatic static index (SSI), is more simple and
practical to perform, and is not so influenced by gait’s velocity, but this method has not yet been adapted to the mouse model of sciatic lesion. We
used 63 male Swiss mice (Mus musculus) to develop a formula to the sciatic static index in mice (SSIm). The animals were divided on three groups
(control, transection and crush). They were evaluated at the preoperative and 7th, 14th, 21st, 28th, 35th and 42nd days postoperative by the ink
track method (SFI), and by the acquisition of photographs of the plantar aspects of the injured and uninjured hind paws. The parameters evaluated
were the 1–5 toe spread (TS), the 2–4 toe spread (ITS) and the distance between the tip of the third toe and the most posterior aspect of the paw
(PL), on both methods. After verifying the temporal pattern of function, correlation and reproducibility of the measurements, we performed a
multiple regression analysis using SFI values as dependent variable, and the TS, ITS and PL measured with the photo method as independent
variables, and found the formula of the SSI for mice (SSIm). The three groups (control, transection and crush) had a characteristic pattern of
dysfunction. The parameters measured in the ink and photo method had variable but significant correlations between them (P < 0.000), but photo
method of measurement showed a better reproducibility. The correlation between SFI and SSIm showed a high correlation coefficient (r = 0.892,
P < 0.000), and demonstrates that SSIm can be used as an alternative method to assess the functional status relative of sciatic nerve activity
in mice. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil / Fundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil / Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Bahiana para Desenvolvimento das Ciências. Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Departamento de Histologia e Embriologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Functional tests | pt_BR |
| Subject | Mice | pt_BR |
| Subject | Sciatic nerve | pt_BR |
| Subject | Sciatic function index | pt_BR |
| Subject | Sciatic static index | pt_BR |
