| Author | Blank, Walter A | pt_BR |
| Author | Reis, Eliana Almeida Gomes | pt_BR |
| Author | Thiong, Fred W | pt_BR |
| Author | Braghiroli, Joao F | pt_BR |
| Author | Santos, Jarbas M | pt_BR |
| Author | Melo, Paulo Roberto Santana | pt_BR |
| Author | Guimarães, Isabel C. S | pt_BR |
| Author | Silva, Luciano Kalabric | pt_BR |
| Author | Carmo, Theomira Mauadie de Azevedo | pt_BR |
| Author | Reis, Mitermayer Galvão dos | pt_BR |
| Author | Blanton, Ronald Edward | pt_BR |
| Access date | 2016-07-05T17:02:47Z | pt_BR |
| Access date | 2016-07-14T19:07:31Z | |
| Available date | 2016-07-05T17:02:47Z | pt_BR |
| Available date | 2016-07-14T19:07:31Z | |
| Document date | 2009 | |
| Citation | BLANK, W. A. et al. Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling. Journal of Parasitology, v. 95, n. 4, p. 881–889, 2009. | pt_BR |
| ISSN | 0022-3395 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14915 | |
| Language | eng | pt_BR |
| Publisher | American Society of Parasitologists | pt_BR |
| Rights | open access | pt_BR |
| Title | Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling | pt_BR |
| Type | Article | pt_BR |
| Abstract | Many parasite populations are difficult to sample because they are non-uniformly distributed between
several host species and are often not easily collected from the living host, limiting sample size and
possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we
investigated the use of the aggregated eggs found in the stool of infected individuals as a simple and
representative sample. Previously, we demonstrated that microsatellite allele frequencies can be
accurately estimated from pooled DNA of cloned S. mansoni adults, and we show here that
genotyping parasite populations from reproductively isolated laboratory strains can be used to
identify these specific populations based on characteristic patterns of allele frequencies, as observed
by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled
PCR products. In addition, microsatellites used to genotype aggregates of eggs collected from stools
of infected individuals produced results consistent with the geographic distribution of the samples.
Direct analysis of total stool eggs can be an important approach to questions of population genetics
for this parasite by increasing the sample size to thousands per individual and reducing bias. | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | University of Nairobi. Nairobi, Kenya | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal da Bahia. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Case Western Reserve University. Center for Global Health and Diseases. Cleveland, Ohio | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Subject | Parasites | pt_BR |
| Subject | Genotype | pt_BR |
| Subject | Microsatellite | pt_BR |
| Subject | Electrophoresis | pt_BR |
| Subject | Genetics, Population | pt_BR |
| Subject | Humans | pt_BR |
