| Author | Quirino, Iara Eliza Pacífico | pt_BR |
| Author | Carneiro, Matheus Batista Heitor | pt_BR |
| Author | Cardoso, Valbert Nascimento | pt_BR |
| Author | Santos, Rosana das Graças Carvalho dos | pt_BR |
| Author | Vieira, Leda Quercia | pt_BR |
| Author | Fiuza, Jacqueline Araújo | pt_BR |
| Author | Alvarez-Leite, Jacqueline Isaura | pt_BR |
| Author | Generoso, Simone de Vasconcelos | pt_BR |
| Author | Correia, Maria Isabel | pt_BR |
| Access date | 2016-06-16T13:15:21Z | pt_BR |
| Access date | 2016-07-14T19:10:32Z | |
| Available date | 2016-06-16T13:15:21Z | pt_BR |
| Available date | 2016-07-14T19:10:32Z | |
| Document date | 2016 | |
| Citation | QUIRINO, Iara Eliza Pacífico et al. Arginine Supplementation Induces Arginase Activity and Inhibits TNF-α Synthesis in Mice Spleen Macrophages After Intestinal Obstruction. Journal of Parenteral and Enteral Nutrition, v. 40, n. 3, p. 417-422, 2016. | pt_BR |
| ISSN | 0148-6071 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/14925 | |
| Language | eng | pt_BR |
| Publisher | American Society for Parenteral and Enteral Nutrition | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Arginine Supplementation Induces Arginase Activity and Inhibits TNF-α Synthesis in Mice Spleen Macrophages After Intestinal Obstruction | pt_BR |
| Type | Article | |
| DOI | 10.1177/0148607114546374 | pt_BR |
| Abstract | Background: The purpose of this study was to assess the effect of arginine supplementation on arginase activity, tumor necrosis factor–α (TNF-α) and interleukin-10 (IL-10) synthesis in cultured splenic macrophages from a murine model of intestinal obstruction (IO). The effects of nitric oxide synthase (iNOS) inhibition were also studied using iNOS knockout animals. Material and Methods: Male C57BL6/J wild-type (WT) and iNOS knockout (iNOS–/–) mice were randomized into 6 groups: Sham and Sham–/– (standard chow), IO and IO–/– (standard chow + IO), and Arg and Arg–/– (standard chow supplemented with arginine + IO). After 7 days of treatment with standard or supplemented chow, IO was induced. Arginase activity as well as TNF-α and IL-10 levels were analyzed in splenic macrophage cultures. Results: Arginine supplementation and the absence of iNOS increased arginase activity in splenic macrophages (Arg, IO–/–, and Arg–/– groups vs the Sham group; P < .05). Arginine was also related to a decrease in TNF-α levels (Arg vs IO group, P < .05) and maintenance of IL-10 levels (Arg vs other groups, P > .05). The inhibition of iNOS did not result in effects on the concentration of cytokines (Sham–/–, IO–/–, and Arg–/– vs other, P < .05). Conclusions: Arginine supplementation and iNOS inhibition led to increased arginase activity. Arginine availability decreased plasma TNF-α levels, which may be directly related to nitric oxide derived from arginine. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Analises Clinicas e Toxicologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Farmácia. Departamento de Analises Clinicas e Toxicologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Renè Rachou. Belo Horizonte, MG, Brasil / Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Enfermagem. Departamento de Nutrição. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Escola de Medicina. Departamento de Cirurgia. Belo Horizonte, MG, Brasil. | pt_BR |
| Subject | Inducible nitric oxide synthase | pt_BR |
| Subject | Arginase | pt_BR |
| Subject | Immune modulation | pt_BR |
