| Autor | Rübsamen-Waigmann, H | |
| Autor | von Briesen, H | |
| Autor | Holmes, H | |
| Autor | Björndal, A | |
| Autor | Korber, B | |
| Autor | Esser, R | |
| Autor | Ranjbar, S | |
| Autor | Tomlinson, P | |
| Autor | Castro Filho, Bernardo Galvão | |
| Autor | Karita, E | |
| Autor | Sempala, S | |
| Autor | Wasi, C | |
| Autor | Osmanov, S | |
| Autor | Fenyo, E.-M | |
| Autor | WHO Network for HIV Isolation and Characterization, WHO Network | |
| Data de acesso | 2016-08-10T13:12:37Z | |
| Data de disponibilização | 2016-08-10T13:12:37Z | |
| Data do publicação | 1994 | |
| Citação | RUBSAMEN-WAIGMANN, H. et al. Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization. AIDS Research and Human Retroviruses, v. 10, n. 11, p. 1401-1408, 1994. | pt_BR |
| ISSN | 0889-2229 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15222 | |
| Idioma | eng | pt_BR |
| Editor | Mary Ann Liebert | pt_BR |
| Direito Autoral | open access | pt_BR |
| Título | Standard conditions of virus isolation reveal biological variability of HIV type 1 in different regions of the world. WHO Network for HIV Isolation and Characterization | pt_BR |
| Tipo do documento | Article | pt_BR |
| Resumo em Inglês | HIV-1 isolates were obtained from four countries within the framework of the WHO Network for HIV
Isolation and Characterization. The use of standard HIV isolation procedures allowed us to compare the biological
properties of 126 HIV-1 isolates spanning five genetic subtypes. In primary isolation cultures, viruses
from Uganda and Brazil appeared early and replicated without delay, whereas the replication of Thai viruses
was delayed by several weeks. Regardless of genetic subtype or country of origin, blood samples collected more
than 2 years after seroconversion yielded virus that replicated efficiently in the primary isolation cultures.
None of the isolates obtained from Thailand or Rwanda replicated in cell lines, whereas 5 of the 13 Brazilian
isolates and 7 of the 11 Ugandan isolates replicated and induced syncytia in Ml -2 cells. As expected for virus
isolates obtained early in HIV-1 infection (within 2 years of seroconversion), all viruses from Brazil, Rwanda,
and Thailand showed a slow/low replicative pattern. For the Ugandan samples, the time from seroconversion
was known precisely for a few of the samples and only in one case was less than 2 years. This may explain why
the five viruses that were able to replicate in all cell lines, and thus classified as rapid/high, were of Ugandan
origin. Viruses able to induce syncytia in MT-2 cells, also induced syncytia in PBMC. However, 8 slow/low
viruses (out of 27) gave discordant results, inducing syncytia in PBMC but not in MT-2 cells. Furthermore, using
syncytium induction as a marker, changes in virus populations during early in vitro passage in PBMC
could be observed. The results indicate that biological variation is a general property of HIV-1 in different regions
of the world. Moreover, the time from HIV-1 infection, rather than genetic subtype, seems to be linked to
viral phenotype. | pt_BR |
| Afiliação | Georg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, Germany | pt_BR |
| Afiliação | Georg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, Germany | pt_BR |
| Afiliação | National Institute of Biological Standards and Control. London, UK | pt_BR |
| Afiliação | Los Alamos National Laboratory. HIV Sequence Database. Los Alamos, New Mexico | pt_BR |
| Afiliação | Georg-Speyer-Haus Chemotherapeutisches Forschunginstitut. Frankfurt, Germany | pt_BR |
| Afiliação | National Institute of Biological Standards and Control. London, UK | pt_BR |
| Afiliação | National Institute of Biological Standards and Control. London, UK | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Afiliação | Institute of Tropical Medicine. Department of Microbiology. Antwerp, Belgium | pt_BR |
| Afiliação | Uganda Virus Research Institute. Entebbe, Uganda | pt_BR |
| Afiliação | Mahidol University. Faculty of Medicine Siriraj Hospital. Department of Microbiology, Division of Virology. Bangkok, Thailand | pt_BR |
| Afiliação | World Health Organization. Global Programme on AIDS. Geneva, Switzerland | pt_BR |
| Afiliação | Los Alamos National Laboratory. HIV Sequence Database. Los Alamos, New Mexico | pt_BR |
| Afiliação | WHO Network for HIV Isolation and Characterization | pt_BR |
| Palavras-chave em inglês | HIV-1 | pt_BR |
| Palavras-chave em inglês | HIV Infections | pt_BR |
| Palavras-chave em inglês | Leukocytes, Mononuclear | pt_BR |
| Palavras-chave em inglês | Phenotype | pt_BR |
| Palavras-chave em inglês | Virus Replication | pt_BR |
| Palavras-chave em inglês | World Health Organization | pt_BR |
| Palavras-chave em inglês | Genetic Variation | pt_BR |
| Palavras-chave em inglês | Genotype | pt_BR |
| Palavras-chave em inglês | Cytopathogenic Effect, Viral | pt_BR |
| Palavras-chave em inglês | Brazil | pt_BR |
| Palavras-chave em inglês | Humans | pt_BR |
