| Autor | Tsunetsugu-Yokota, Yasuko | |
| Autor | Morikawa, Yuko | |
| Autor | Isogai, Maya | |
| Autor | Kawana-Tachikawa, Ai | |
| Autor | Odawara, Takashi | |
| Autor | Nakamura, Tetsuya | |
| Autor | Grassi, Maria Fernanda Rios | |
| Autor | Autran, Brigitte | |
| Autor | Iwamoto, Aikichi | |
| Data de acesso | 2016-08-11T16:15:38Z | |
| Data de disponibilização | 2016-08-11T16:15:38Z | |
| Data do publicação | 2003 | |
| Citação | TSUNETSUGU-YOKOTA, Y. et al. Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs. Journal of Virology, v. 77, n. 19, p. 10250–10259, 2003. | pt_BR |
| ISSN | 0022-538X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15242 | |
| Idioma | eng | pt_BR |
| Editor | American Society for Microbiology | pt_BR |
| Direito Autoral | open access | pt_BR |
| Título | Yeast-derived human immunodeficiency virus type 1 p55(gag) virus-like particles activate dendritic cells (DCs) and induce perforin expression in Gag-specific CD8(+) T cells by cross-presentation of DCs | pt_BR |
| Tipo do documento | Article | pt_BR |
| Resumo em Inglês | To evaluate the immunogenicity of human immunodeficiency virus (HIV) type 1 p55(gag) virus-like particles (VLPs) released by budding from yeast spheroplasts, we have analyzed the effects of yeast VLPs on monocyte-derived dendritic cells (DCs). Yeast VLPs were efficiently incorporated into DCs via both macropinocytosis and endocytosis mediated by mannose-recognizing receptors, but not the mannose receptor. The uptake of yeast VLPs induced DC maturation and enhanced cytokine production, notably, interleukin-12 p70. We showed that yeast membrane components may contribute to DC maturation partly through Toll-like receptor 2 signaling. Thus, Gag particles encapsulated by yeast membrane may have an advantage in stimulating Gag-specific immune responses. We found that yeast VLPs, but not the control yeast membrane fraction, were able to activate both CD4(+) and CD8(+) T cells of HIV-infected individuals. We tested the effect of cross-presentation of VLP by DCs in two subjects recruited into a long-term nonprogressor-slow progressor cohort. When yeast VLP-loaded DCs of these patients were cocultured with peripheral blood mononuclear cells for 7 days, approximately one-third of the Gag-specific CD8(+) T cells were activated and became perforin positive. However, some of the Gag-specific CD8(+) T cells appeared to be lost during in vitro culture, especially in a patient with a high virus load. Our results suggest that DCs loaded with yeast VLPs can activate Gag-specific memory CD8(+) T cells to become effector cells in chronically HIV-infected individuals, but there still remain unresponsive Gag-specific T-cell populations in these patients. | pt_BR |
| Afiliação | National Institute of Infectious Diseases. Department of Immunology. Shinjuku-ku | pt_BR |
| Afiliação | Kitasato University. Kitasato Institute for Life Sciences | pt_BR |
| Afiliação | National Institute of Infectious Diseases. Department of Immunology. Shinjuku-ku | pt_BR |
| Afiliação | University of Tokyo. Institute of Medical Science. Department of Infectious Diseases. Tokyo | pt_BR |
| Afiliação | University of Tokyo. Institute of Medical Science. Department of Infectious Diseases. Tokyo | pt_BR |
| Afiliação | University of Tokyo. Institute of Medical Science. Department of Infectious Diseases. Tokyo | pt_BR |
| Afiliação | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, Brasil | pt_BR |
| Afiliação | Hôpital Pitié-Salpêtrière. Laboratoire d’Immunologie Cellulaire et Tissulaire. Paris, France | pt_BR |
| Afiliação | University of Tokyo. Institute of Medical Science. Department of Infectious Diseases. Tokyo | pt_BR |
| Palavras-chave em inglês | HIV-1 | pt_BR |
| Palavras-chave em inglês | Dendritic Cells | pt_BR |
| Palavras-chave em inglês | Interleukin-12 | pt_BR |
| Palavras-chave em inglês | CD8-Positive T-Lymphocytes | pt_BR |
| Palavras-chave em inglês | Interferon-gamma | pt_BR |
| Palavras-chave em inglês | Membrane Glycoproteins | pt_BR |
| Palavras-chave em inglês | Humans | pt_BR |
| Palavras-chave em inglês | Protein Precursors | pt_BR |
| e-ISSN | 10.1128/JVI.77.19.10250–10259.2003 | |
