| Author | Costa, Eliane Veiga da | |
| Author | Campos, Renata de Mendonça | |
| Author | Tavares, Fernando Neto | |
| Author | Grégio, Cátia Regina Valério | |
| Author | Burlandy, Fernanda Marcicano | |
| Author | Silva, Edson Elias da | |
| Access date | 2016-08-23T11:42:36Z | |
| Available date | 2016-08-23T11:42:36Z | |
| Document date | 2012 | |
| Citation | COSTA, Eliane Veiga da; et al. A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures. Mem Inst Oswaldo Cruz, v.107, n.5, p.698-701, Aug. 2012. | pt_BR |
| ISSN | 1678-8080 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15325 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Poliomielite | pt_BR |
| Subject in Portuguese | Vacina Antipólio | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Title | A RT-PCR method for selective amplification and phenotypic characterization of all three serotypes of Sabin-related polioviruses from viral mixtures | pt_BR |
| Type | Article | |
| DOI | 10.1590/S0074-02762012000500022 | |
| Abstract | Outbreaks caused by vaccine-derived polioviruses are challenging the final eradication of paralytic poliomyelitis.
Therefore, the surveillance of the acute flaccid paralysis cases based on poliovirus isolation and characterization
remains an essential activity. Due to the use of trivalent oral poliovirus vaccine (OPV), mixtures containing
more than one serotype of Sabin-related polioviruses are frequently isolated from clinical samples. Because each
poliovirus isolate needs to be individually analyzed, we designed polymerase chain reaction primers that can selectively
distinguish and amplify a genomic segment of the three Sabin-related poliovirus serotypes present in mixtures,
thus, optimizing the diagnosis and providing prompt information to support epidemiologic actions. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Diagnostic techniques and procedures | pt_BR |
| Subject | VP1 protein | pt_BR |
| Subject | Poliovirus | pt_BR |
| Subject | RT-PCR method | pt_BR |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
