| Author | Allonso, Diego | |
| Author | Rosa, Marcela da Silva | |
| Author | Coelho, Diego Rodrigues | |
| Author | Costa, Simone Morais da | |
| Author | Nogueira, Rita Maria Ribeiro | |
| Author | Bozza, Fernando A. | |
| Author | Santos, Flávia Barreto dos | |
| Author | Alves, Ada Maria de Barcelos | |
| Author | Mohana-Borges, Ronaldo | |
| Access date | 2016-08-24T11:35:23Z | |
| Available date | 2016-08-24T11:35:23Z | |
| Document date | 2011 | |
| Citation | ALLONSO, Diego et al. Polyclonal antibodies against properly folded Dengue virus NS1 protein expressed in E. coli enable sensitive and early dengue diagnosis. Journal of Virological Methods, v. 175, n. 1, p. 109-116, July 2011. | pt_BR |
| ISSN | 0166-0934 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15358 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Vírus da Dengue | pt_BR |
| Subject in Portuguese | Dengue | pt_BR |
| Subject in Portuguese | Diagnóstico | pt_BR |
| Subject in Portuguese | Escherichia coli | pt_BR |
| Title | Polyclonal antibodies against properly folded Dengue virus NS1 protein expressed in E. coli enable sensitive and early dengue diagnosis | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jviromet.2011.04.029 | |
| Abstract | The non-structural 1 (NS1) protein plays an important role in dengue diagnosis because it has been detected as a soluble serum antigen in both primary and secondary infections. The NS1 protein was expressed in Escherichia coli cells, and the efficiency of four different refolding protocols was tested. All of the protocols generated dimeric NS1 in a conformation similar to that of the protein expressed by eukaryotic cells. A polyclonal antibody produced from the properly folded E. coli recombinant NS1 (rNS1) protein proved to be a useful tool for the diagnosis of Dengue virus because it detected 100% of the Dengue virus 2 (DENV2) in infected patients' sera and 60% of the DENV IgM-positive sera not detected by commercial NS1-based diagnostic kits. These data suggest a high-efficiency method for correctly folding rNS1 that maintains its structural and immunogenic properties. In addition, a detection method using the polyclonal antibody against correctly folded rNS1 seemed to be more sensitive and efficient for NS1 detection in serum, highlighting its usefulness for developing a high-sensitivity diagnostic kit. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Genômica Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Genômica Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia das Infecções Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto de Pesquisas Clínicas Evandro Chagas. Unidade de Terapia Intensiva. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Flavivírus. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biotecnologia e Fisiologia das Infecções Virais. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Genômica Estrutural. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Dengue virus | pt_BR |
| Subject | NS1 protein | pt_BR |
| Subject | Dengue diagnosis | pt_BR |
| Subject | E. coli | pt_BR |
