| Author | Almeida, Fabiana Greyce Oliveira | pt_BR |
| Author | Aquino, Priscila Ferreira de | pt_BR |
| Author | Souza, Afonso Duarte Leão de | pt_BR |
| Author | Souza, Antonia Queiroz Lima de | pt_BR |
| Author | Vinhote, Sonia do Carmo | pt_BR |
| Author | Mac-Cormick, Thaís Messias | pt_BR |
| Author | Silva Marcelo Soares da Mota | pt_BR |
| Author | Chalub, Sidney Raimundo Silva | pt_BR |
| Author | Fischer, Juliana de Saldanha da Gama | pt_BR |
| Author | Carvalho, Paulo Costa | pt_BR |
| Author | Carvalho, Maria da Gloria da Costa | pt_BR |
| Access date | 2016-08-26T16:31:19Z | pt_BR |
| Available date | 2016-08-26T16:31:19Z | pt_BR |
| Document date | 2015 | |
| Citation | Almeida FGO, de Aquino PF, de Souza ADL, et al. Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil. Biological Research, v. 48, n. 1, p. 50, 2015. | pt_BR |
| ISSN | 0716-9760 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15494 | |
| Sponsorship | CAPES, CNPq, Cancer Foundation (Oncobiologia Grant), and FAPEAM | pt_BR |
| Language | por | pt_BR |
| Rights | open access | pt_BR |
| Title | Colorectal cancer DNA methylation patterns from patients in Manaus, Brazil | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1186/s40659-015-0042-7 | pt_BR |
| Abstract | Background
DNA methylation is commonly linked with the silencing of the gene expression for many tumor suppressor genes. As such, determining DNA methylation patterns should aid, in times to come, in the diagnosis and personal treatment for various types of cancers. Here, we analyzed the methylation pattern from five colorectal cancer patients from the Amazon state in Brazil for four tumor suppressor genes, viz.: DAPK, CDH1, CDKN2A, and TIMP2 by employing a polymerase chain reaction (PCR) specific to methylation. Efforts in the study of colorectal cancer are fundamental as it is the third most of highest incidence in the world.
Results
Tumor biopsies were methylated in 1/5 (20 %), 2/5 (40 %), 4/5 (80 %), and 4/5 (80 %) for CDH1, CDKN2A, DAPK, and TIMP2 genes, respectively. The margin biopsies were methylated in 3/7 (43 %), 2/7 (28 %), 7/7 (100 %), and 6/7 (86 %) for CDH1, CDKN2A, DAPK, and TIMP2, respectively.
Conclusions
Our findings showed DAPK and TIMP2 to be methylated in most samples from both tumor tissues and adjacent non-neoplastic margins; thus presenting distinct methylation patterns. This emphasizes the importance of better understanding of the relation of these patterns with cancer in the context of different populations. | pt_BR |
| Affilliation | Federal University of Amazonas. Institute of Exact Sciences. Laboratory of Chromatography and Mass Spectrometry. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Oswaldo Cruz Fundation. Instituto Leonidas e Maria Deane. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Amazon State University. High School of Health. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Federal University of Rio de Janeiro. Department of Pathology. Laboratory of Molecular Pathology. Rio de Janeiro, RJ, Brazil. | pt_BR |
| Affilliation | Oncology Control Center Foundation of Amazon State. Department of Abdominal Surgery. Manaus, AM, Brazil. | pt_BR |
| Affilliation | Oswaldo Cruz Fundation. Carlos Chagas Institute. Laboratory of Proteomics and Protein Engineering. Curitiba, PR, Brazil. | pt_BR |
| Subject | Methylation | pt_BR |
| Subject | Colorectal cancer | pt_BR |
| Subject | Amazon | pt_BR |
| Subject | Epigenetic | pt_BR |
