| Author | Morais, Mariana Abrahão Bueno de | |
| Author | Souza, Tatiana de Arruda Campos Brasil de | |
| Author | Murakami, Mario Tyago | |
| Access date | 2016-09-02T17:57:41Z | |
| Available date | 2016-09-02T17:57:41Z | |
| Document date | 2013 | |
| Citation | MORAIS, Mariana Abrahão Bueno de et al. Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis. Acta Crystallogr. Sect F. Struct. Biol. Cryst. Commun, n. 69, p. 408-411. | pt_BR |
| ISSN | 1744-3091 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15600 | |
| Sponsorship | This research was supported by grants from Fundação de Amparo a Pesquisa do Estado de São Paulo (FAPESP), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Coordenação
de Aperfeiçoamento de Pessoal de Nível Superior (CAPES). | pt_BR |
| Language | por | pt_BR |
| Rights | open access | pt_BR |
| Title | Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the mitochondrial tryparedoxin peroxidase from Leishmania braziliensis | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1107/S1744309113003989 | pt_BR |
| Abstract | Tryparedoxin peroxidase (TXNPx) is an essential constituent of the main enzymatic scavenger system for reactive oxygen species (ROS) in trypanosomatids. Genetic studies have demonstrated the importance of this system for the development and virulence of these parasites, representing a potential target for the discovery of new trypanocidal drugs. In this work, the mitochondrial TXNPx from Leishmania braziliensis was cloned, overexpressed, purified and crystallized. The crystals diffracted to 3.3 Å resolution and belonged to space group P4(2)2(1)2, with unit-cell parameters a = b = 131.8, c = 44.4 Å. These studies will contribute to a better understanding of the molecular mechanisms involved in ROS detoxification by trypanosomatids. | pt_BR |
| Affilliation | Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil. | pt_BR |
| Affilliation | Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil. / Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Luz Síncrotron. Campinas, SP, Brasil. | pt_BR |
| Subject | Leishmania braziliensis | pt_BR |
| Subject | tryparedoxin peroxidase | pt_BR |
| DeCS | Crystallization | En |
| DeCS | Macromolecular Substances | En |
| DeCS | Crystallography | En |
| DeCS | Cristalização | pt_BR |
| DeCS | Substâncias Macromoleculares | pt_BR |
| DeCS | Cristalografia | pt_BR |
