| Author | Marcolino, Monica Mendes Kangussu | |
| Author | Cunha, Arildo Pinto da | |
| Author | Ávila, Andréa Rodrigues | |
| Author | Herman, Jean-Paul | |
| Author | Rocha, Wanderson Duarte da | |
| Access date | 2016-09-14T13:56:43Z | |
| Available date | 2016-09-14T13:56:43Z | |
| Document date | 2015 | |
| Citation | MARCOLINO, Monica Mendes Kangussu et al. Conditional removal of selectable markers in Trypanosoma cruzi using a site-specific recombination tool: proof of concept. Molecular and Biochemical Parasitology, v. 198, n. 2, p. 71-74, 2015. | pt_BR |
| ISSN | 0166-6851 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15790 | |
| Sponsorship | CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) e Fundação Araucária. | pt_BR |
| Language | por | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Conditional removal of selectable markers in Trypanosoma cruzi using a site-specific recombination tool: proof of concept | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.molbiopara.2015.01.001 | pt_BR |
| Abstract | The scarcity of molecular tools for genetic manipulation is a critical obstacle for functional genomics studies on Trypanosoma cruzi. The current study adapted an inducible site-specific recombination system based on Dimerizable CRE recombinase (DiCRE). Two vectors for stable transfection were created, a first one to express inactive portions of DiCRE recombinase, and a second plasmid containing the loxP sites to test DiCRE activity. After integrating both constructs into the T. cruzi genome, it was shown that DiCRE recombinase can be efficiently used to manipulate its genome by allowing the removal of selectable markers thus generating homogeneous populations. The DiCRE recombinase success allows conditional knockout and the removal of selectable markers without prior parasite modification, which also facilitate the transferring of DiCRE recombinase to different T. cruzi strains.y CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico) and Fundação Araucária. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | National Centre for Scientific Research. Paris, France / Aix-Marseille University. Marseille, France. | pt_BR |
| Affilliation | Universidade Federal do Paraná. Departamento de Bioquímica e Biologia Molecular. Curitiba, PR, Brasil. | pt_BR |
| Subject | DiCRE | pt_BR |
| Subject | loxP | pt_BR |
| Subject | Selectable markers removal | pt_BR |
| Subject | Conditional knockout | pt_BR |
| Subject | Chagas disease | pt_BR |
