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AutorFoti, Leonardo
AutorSionek, Andre
AutorStori, Elis Moura
AutorSoares, Paula Poli
AutorPereira, Miriam Marzall
AutorKrieger, Marco Aurélio
AutorPetzhold, Cesar Liberato
AutorSchreiner, Wido Herwig
AutorSoares, Maurilio José
AutorGoldenberg, Samuel
AutorSaul, Cyro Ketzer
Fecha de acceso2016-09-14T16:43:05Z
Fecha de disponibilización2016-09-14T16:43:05Z
Fecha de publicación2015
ReferenciaFOTI, Leonardo et al. Electrospray induced surface activation of polystyrene microbeads for diagnostic applications. Journal of Materials Chemistry B, n. 3, p. 2725-2731, 2015.pt_BR
ISSN2050-750Xpt_BR
URIhttps://www.arca.fiocruz.br/handle/icict/15792
PromociónINCT para Diagnósticos em Saúde Pública (National Institute of Science and Technology for Diagnostics in Public Health), funded by the National Council of Technological and Scientific Development (CNPq – Brasil)pt_BR
Idiomaporpt_BR
EditorChristine Schmidtpt_BR
Derechos de autorrestricted accesspt_BR
TítuloElectrospray induced surface activation of polystyrene microbeads for diagnostic applicationspt_BR
Tipo del documentoArticle
DOI10.1039/c4tb01884bpt_BR
Resumen en InglésElectrospray is generally regarded as a “soft” technique due to the absence of any observable molecular fragmentation or destruction. This study reports on a novel and easy way to induce surface activation on the surface of polystyrene microbeads through electrospray deposition into a grounded aqueous electrolyte solution bath. This process, nicknamed EISA, which stands for electrospray induced surface activation, proposes that when a highly charged microbead formed by the electrospray process sinks into the aqueous electrolyte solution, it behaves like a highly charged spherical capacitor that discharges in the conductive liquid. The energy released leads to a breakup of the polystyrene surface bonds and water oxidation with oxygen. Further reactions produce a carboxylated surface that was confirmed by X-ray photoelectron spectroscopy (XPS) and protein coupling. An immunoassay based on these modified microbeads was also developed and presented for use in syphilis detection, demonstrating a reliable signal-to-noise ratio between positive and negative results.pt_BR
AfiliaciónInstituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.pt_BR
AfiliaciónUniversidade Federal do Paraná. Departamento de Física. Curitiba, PR, Brasil.pt_BR
AfiliaciónUniversidade Federal do Rio Grande do Sul. Instituto de Física. Porto Alegre, RS, Brasil.pt_BR
AfiliaciónUniversidade Federal do Rio Grande do Sul. Instituto de Química. Porto Alegre, RS, Brasil.pt_BR
AfiliaciónInstituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.pt_BR
AfiliaciónInstituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.pt_BR
AfiliaciónUniversidade Federal do Rio Grande do Sul. Instituto de Química. Porto Alegre, RS, Brasil.pt_BR
AfiliaciónUniversidade Federal do Paraná. Departamento de Física. Curitiba, PR, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.pt_BR
AfiliaciónFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.pt_BR
AfiliaciónUniversidade Federal do Paraná. Departamento de Física. Curitiba, PR, Brasil.pt_BR
Palavras clave en InglêsElectrospraypt_BR
Palavras clave en InglêsX-ray photoelectron spectroscopypt_BR
Palavras clave en InglêsPolystyrenept_BR
Palavras clave en InglêsSyphilispt_BR


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