Author | Souza, Thiago Moreno L. | |
Author | Rodrigues, Diego Q. | |
Author | Passaes, Caroline P. B. | |
Author | Souza, Victor Barreto de | |
Author | Aguiar, Renato S. | |
Author | Temerozo, Jairo R. | |
Author | Morgado, Mariza G. | |
Author | Fontes, Carlos Frederico L. | |
Author | Araujo, Elizabeth G. | |
Author | Bou-Habib, Dumith Chequer | |
Access date | 2016-09-20T16:59:14Z | |
Available date | 2016-09-20T16:59:14Z | |
Document date | 2011 | |
Citation | SOUZA, Tiago Moreno L. et al. The nerve growth factor reducesAPOBEC3G synthesis and enhances HIV-1 transcription and replication in human primary macrophages. Blood, v.117, n.10, p.2944-2952, Mar. 2011. | pt_BR |
ISSN | 0006-4971 | pt_BR |
URI | https://www.arca.fiocruz.br/handle/icict/15872 | |
Language | eng | pt_BR |
Publisher | The American Society of Hematology | pt_BR |
Rights | restricted access | pt_BR |
Subject in Portuguese | HIV-1 | pt_BR |
Subject in Portuguese | Infecção por HIV-1 | pt_BR |
Subject in Portuguese | Macrófagos | pt_BR |
Subject in Portuguese | Replicação viral | pt_BR |
Subject in Portuguese | Imunopatogenia | pt_BR |
Subject in Portuguese | Fator de Crescimento Neural | pt_BR |
Title | The nerve growth factor reduces APOBEC3G synthesis and enhances HIV-1 transcription and replication in human primary macrophages | pt_BR |
Type | Article | pt_BR |
DOI | 10.1182/blood-2010-05-287193 | |
Abstract | Macrophages infected with HIV-1 sustain viral replication for long periods of time, functioning as viral reservoirs. Therefore, recognition of factors that maintain macrophage survival and influence HIV-1 replication is critical to understanding the mechanisms that regulate the HIV-1-replicative cycle. Because HIV-1-infected macrophages release the nerve growth factor (NGF), and NGF neutralization reduces viral production, we further analyzed how this molecule affects HIV-1 replication. In the present study, we show that NGF stimulates HIV-1 replication in primary macrophages by signaling through its high-affinity receptor Tropomyosin-related Kinase A (TrKA), and with the involvement of reticular calcium, protein kinase C, extracellular signal-regulated kinase, p38 kinase, and nuclear factor-κB. NGF-induced enhancement of HIV-1 replication occurred during the late events of the HIV-1-replicative cycle, with a concomitant increase in viral transcription and production. In addition, NGF reduced the synthesis of the cellular HIV-1 restriction factor APOBEC3G and also overrode its interferon-γ-induced up-regulation, allowing the production of a well-fitted virus. Because NGF-TrKA signaling is a crucial event for macrophage survival, it is possible that NGF-induced HIV-1 replication plays a role in the maintenance of HIV-1 reservoirs. Our study may contribute to the understanding of the immunopathogenesis of HIV-1 infection and provide insights about approaches aimed at limiting viral replication in HIV-1 reservoirs. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Estrutura e Regulação de Proteínas e ATPases. Rio de Janeiro, RJ., Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biologia. Departamento de Genética. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. | pt_BR |
Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica. Laboratório de Estrutura e Regulação de Proteínas e ATPases. Rio de Janeiro, RJ., Brasil. | pt_BR |
Affilliation | Universidade Federal Fluminense. Laboratório de Cultura de Tecidos Hertha Meyer. Niterói, RJ, Brasil. | pt_BR |
Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo Rio de Janeiro, RJ, Brasil. | pt_BR |
Subject | HIV-1 | pt_BR |
Subject | Macrophages | pt_BR |
Subject | HIV-1 infection | pt_BR |
Subject | Immunopathogenesis | pt_BR |
Subject | Viral replication | pt_BR |
Subject | Nerve Growth Factor | pt_BR |
e-ISSN | 1528-0020 | |
Embargo date | 2030-01-01 | |