| Author | Paiva Neto, Claúdia | |
| Author | Pyrrho, Alexandre dos Santos | |
| Author | Vieira, Joseli Lannes | |
| Author | Vacchio, Melanie | |
| Author | Soares, Milena Botelho Pereira | |
| Author | Gattass, Cerli Rocha | |
| Access date | 2016-09-27T13:21:11Z | |
| Available date | 2016-09-27T13:21:11Z | |
| Document date | 2003 | |
| Citation | PAIVA NETO, C. et al. Trypanosoma Cruzi sensitizes mice to fulminant seb-induced Shock: Overrelease of Iinflammatory cytokines and independence of Chagas’ Disease or TCR Vb-Usage. Shock, v. 19, n. 2, p. 163–168, 2003. | pt_BR |
| ISSN | 1073-2322 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15958 | |
| Sponsorship | Programa de Apoio a Núcleos de Excelência Conselho Nacional de Pesquisas. Fundação de Amparo a Pesquisa de Estado do Rio de Janeiro. World Health Organization | pt_BR |
| Language | eng | pt_BR |
| Publisher | Lippincott, Williams & Wilkins | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | Repertório | pt_BR |
| Subject in Portuguese | Clone CL-14 | pt_BR |
| Subject in Portuguese | Cepa CL | pt_BR |
| Subject in Portuguese | Enterotoxina B de estafilococos | pt_BR |
| Subject in Portuguese | Sepsia | pt_BR |
| Subject in Portuguese | Linfócitos T | pt_BR |
| Subject in Portuguese | TNF-a | pt_BR |
| Subject in Portuguese | IFN-g | pt_BR |
| Title | Trypanosoma Cruzi sensitizes mice to fulminant seb-induced Shock: Overrelease of Iinflammatory cytokines and independence of Chagas’ Disease or TCR Vb-Usage | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1097/01.SHK.0000054747.80210.e9 | |
| Abstract | Trypanosoma cruzi–infected mice display increased susceptibility to shock induced by injection of lipopolysaccharide
(LPS), anti-CD3, or resulting from interleukin (IL)-10-defective response to the parasite itself, but the basis of
such susceptibility remains unknown. Herein, we tested the susceptibility of mice inoculated with virulent and avirulent T.
cruzi to staphylococcal enterotoxins (SE), potent inducers of inflammatory cytokine secretion. Mice infected with T. cruzi
CL-strain or inoculated with the avirulent clone CL-14, a clone that does not induce disease or polyclonal lymphocyte
activation, succumb suddenly to low doses of staphylococcal enterotoxin B (SEB), but not to staphylococcal enterotoxin
A (SEA). High plasma levels of TNF, IFN-g, and liver transaminases alanine aminotransferase (ALT) and aspartate
aminotransferase (AST) were found in these mice, indicating lethal toxic shock. Sensitization to shock required inoculation
of live avirulent trypomastigotes and a time interval before challenge with SEB. We found no prior skewing of T cell
receptor (TCR) Vb-repertoire in CL-14-inoculated mice that could be responsible for sensitization. Splenocytes from
CL-14-inoculated mice proliferated more under anti-Vb8 than anti-TCRb stimulation when compared with normal mice, but
were suppressed to SEB stimulation. Both SEB and anti-Vb8 antibodies stimulated splenocytes from T. cruzi-inoculated
mice to secrete higher levels of inflammatory cytokines than normal controls. Taken together, our results show that T. cruzi
inoculation can sensitize mice to lethal SEB-induced shock even in the absence of tissue damage, polyclonal lymphocyte
activation, or previously increased levels of inflammatory cytokines, and they suggest that altered reactivity of Vb8
lymphocytes may be involved in the phenomenon | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Departamento de Análises Clinicas e Toxicológicus. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | Instituto Oswaldo Cruz. Fundação Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil | pt_BR |
| Affilliation | National Cancer Institute. National Institutes of Health. Experimental Immunology Branch. Bethesda, Maryland | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil | pt_BR |
| Subject | Repertoire | pt_BR |
| Subject | Clone CL-14 | pt_BR |
| Subject | CL strain | pt_BR |
| Subject | Staphylococcal enterotoxin B | pt_BR |
| Subject | Sepsis | pt_BR |
| Subject | T lymphocyte | pt_BR |
| Subject | TNF-a | pt_BR |
| Subject | IFN-g | pt_BR |
