| Author | Assis, Rafael Ramiro de | |
| Author | Ludolf, Fernanda | |
| Author | Nakajima, Rie | |
| Author | Jasinskas, Al | |
| Author | Oliveira, Guilherme Correa de | |
| Author | Felgner, Philip L. | |
| Author | Jangola, Soraya Torres Gaze | |
| Author | Loukas, Alex | |
| Author | LoVerde, Philip T. | |
| Author | Bethony, Jeffrey Michael | |
| Author | Oliveira, Rodrigo Correa de | |
| Author | Silva, Carlos Eduardo Calzavara | |
| Access date | 2016-09-28T17:58:58Z | |
| Available date | 2016-09-28T17:58:58Z | |
| Document date | 2016 | |
| Citation | ASSIS, Rafael Ramiro de Assis, et al. A next-generation proteome array for Schistosoma mansoni. Int J Parasitol., v. 46, n. 7, p. 411-5, 2016. | pt_BR |
| ISSN | 0020-7519 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/15989 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Ltd. | pt_BR |
| Rights | open access | pt_BR |
| Subject in Portuguese | anticorpos | pt_BR |
| Subject in Portuguese | proteomes | pt_BR |
| Title | A next-generation proteome array for Schistosoma mansoni | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1016/j.ijpara.2016.04.001 | |
| Abstract | A proteome microarray consisting of 992 Schistosoma mansoni proteins was produced and screened with sera to determine antibody signatures indicative of the clinical stages of schistosomiasis and the identification of subunit vaccine candidates. Herein, we describe the methods used to derive the gene list for this array (representing approximately 10% of the predicted S. mansoni proteome). We also probed a pilot version of the microarray with sera from individuals either acutely or chronically infected with S. mansoni from endemic areas in Brazil and sera from individuals resident outside the endemic area (USA) to determine if the array is functional and informative. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/ University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Infectologia e Medicina Tropical. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA | pt_BR |
| Affilliation | University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA | pt_BR |
| Affilliation | Vale Technology Institute of Sustainable Development. Belém, PA, Brazil | pt_BR |
| Affilliation | University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | James Cook University. Australian Institute of Tropical Health & Medicine. Centre for Biodiscovery and Molecular Development of Therapeutics. Cairns, QLD, Australia | pt_BR |
| Affilliation | University of Texas Health Science Center. School of Medicine. Departments of Biochemistry and Pathology. San Antonio, TX , USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/The George Washington University. School of Medicine and Health Science. Department of Microbiology, Immunology, and Tropical Medicine. Washington, DC, USA | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisa Rene Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brazil/ University of California. School of Medicine. Division of Infectious Disease. Protein Microarray Laboratory. Irvine, CA, USA | pt_BR |
| Subject | Antibody signature | pt_BR |
| Subject | Protein microarray | pt_BR |
| Subject | Proteome | pt_BR |
| Subject | Schistosoma mansoni | pt_BR |
| Embargo date | 2021-01-01 | |
