| Author | Inoue, Alexandre Haruo | |
| Author | Serpeloni, Mariana | |
| Author | Hiraiwa, Priscila Mazzocchi | |
| Author | Yamada-Ogatta, Sueli Fumie | |
| Author | Muniz, João Renato Carvalho | |
| Author | Motta, Maria Cristina Machado | |
| Author | Vidal, Newton Medeiros | |
| Author | Goldenberg, Samuel | |
| Author | Ávila, Andréa Rodrigues | |
| Access date | 2016-10-03T16:43:16Z | |
| Available date | 2016-10-03T16:43:16Z | |
| Document date | 2014 | |
| Citation | INOUE, Alexandre Haruo et al. Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes. PLoS ONE, v. 9, n. 10, p. 1-11, Oct. 2014. | pt_BR |
| ISSN | 1932-6203 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16050 | |
| Sponsorship | Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nıvel Superior (CAPES), Fundação Oswaldo Cruz (PAPES-FIOCRUZ Program) and Fundação Araucaria-PR. | pt_BR |
| Language | eng | pt_BR |
| Publisher | Public Library of Science | pt_BR |
| Rights | open access | pt_BR |
| Title | Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes | pt_BR |
| Type | Article | pt_BR |
| DOI | 10.1371/journal.pone.0109521 | pt_BR |
| Abstract | Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Paraná. Laboratório de Biologia Celular e Molecular. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina, PR, Brasil. | pt_BR |
| Affilliation | Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Departamento de Biologia Celular e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / National Institutes of Health. National Library of Medicine. National Center for Biotechnology Information. Bethesda, Maryland, USA. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Subject | RNA helicase | pt_BR |
| Subject | Trypanosomes | pt_BR |
| Subject | Nucleocytoplasmic shuttling | pt_BR |
| Subject | Gene Expression | En |
| DeCS | Expressão Gênica | pt_BR |
| DeCS | Transporte Ativo do Núcleo Celular | pt_BR |
| DeCS | Trypanosoma | pt_BR |
