| Author | Guimarães, Gabriel | |
| Author | Souto, Dênio Emanuel Pires | |
| Author | Assis, Girley Francisco Machado | |
| Author | Lana, Marta de | |
| Author | Luz, Rita de Cassia Silva | |
| Author | Martins Filho, Olindo Assis | |
| Author | Damos, Flávio Santos | |
| Author | Martins, Helen Rodrigues | |
| Access date | 2016-10-05T18:58:29Z | |
| Available date | 2016-10-05T18:58:29Z | |
| Document date | 2016 | |
| Citation | GUIMARÃES, Gabriel et al. Applicability of a novel immunoassay based on surface plasmon resonance for the diagnosis of Chagas disease. Clin Chim Acta, vol. 454, p. 39-45, 2016. | pt_BR |
| ISSN | 0009-8981 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16106 | |
| Language | eng | pt_BR |
| Publisher | Elsevier Scientific Pub. Co | pt_BR |
| Rights | restricted access | pt_BR |
| Subject in Portuguese | Doença de chagas | pt_BR |
| Title | Applicability of a novel immunoassay based on surface plasmon resonance for the diagnosis of Chagas disease | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.cca.2015.12.025 | |
| Abstract | BACKGROUND: We defined the methodological criteria for the interpretation of the results provided by a novel immunoassay based on surface plasmon resonance (SPR) to detect antibodies anti-Trypanosoma cruzi in human sera (SPRCruzi). Then, we evaluated its applicability as a diagnostic tool for Chagas disease.
METHODS: To define the cut-off point and serum dilution factor, 57 samples were analyzed at SPRCruzi and the obtained values of SPR angle displacement (ΔθSPR) were submitted to statistical analysis. Adopting the indicated criteria, its performance was evaluated into a wide panel of samples, being 99 Chagas disease patients, 30 non-infected subjects and 42 with other parasitic/infectious diseases. In parallel, these samples were also analyzed by ELISA.
RESULTS: Our data demonstrated that 1:320 dilution and cut-off point at ∆θSPR=17.2 m° provided the best results. Global performance analysis demonstrated satisfactory sensitivity (100%), specificity (97.2%), positive predictive value (98%), negative predictive value (100%) and global accuracy (99.6%). ELISA and SPRCruzi showed almost perfect agreement, mainly between chagasic and non-infected individuals. However, the new immunoassay was better in discriminate Chagas disease from other diseases.
CONCLUSION: This work demonstrated the applicability of SPRCruzi as a feasible, real time, label free, sensible and specific methodology for the diagnosis of Chagas disease. | pt_BR |
| Affilliation | Universidade Federal de Mato Grosso. Instituto de Ciências Exatas e Naturais. Rondonópolis, MT, Brasil/Universidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Diamantina, MG, Brasil | pt_BR |
| Affilliation | Universidade Estadual de Campinas. Instituto Nacional de Ciência e Tecnologia em Bioanalítica. Campinas, SP, Brasil | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Laboratório de Parasitologia. Governador Valadares, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Doenças de Chagas. Ouro Preto, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Departamento de Química. São Luís, MA, Brasil | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, Brasil | pt_BR |
| Affilliation | Universidade Federal do Maranhão. Departamento de Química. São Luís, MA, Brasil | pt_BR |
| Affilliation | Universidade Federal dos Vales do Jequitinhonha e Mucuri. Departamento de Farmácia. Diamantina, MG, Brasil | pt_BR |
| Subject | Applicability | pt_BR |
| Subject | Chagas disease | pt_BR |
| Subject | Diagnosis | pt_BR |
| Subject | Immunoassay | pt_BR |
| Subject | Surface plasmon resonance | pt_BR |
| Embargo date | 2022-01-01 | |
| xmlui.metadata.dc.subject.ods | 03 Saúde e Bem-Estar | |
