| Author | Rocha, Adalgiza da Silva | |
| Author | Cunha, Maria das Graças | |
| Author | Diniz, Lucia Martins | |
| Author | Salgado, Claudio | |
| Author | Aires, Maria Araci P. | |
| Author | Nery, José Augusto | |
| Author | Gallo, Eugênia Novisck | |
| Author | Miranda, Alice | |
| Author | Magnanini, Monica M. F. | |
| Author | Matsuoka, Masanori | |
| Author | Sarno, Euzenir Nunes | |
| Author | Suffys, Philip Noel | |
| Author | Oliveira, Maria Leide W. de | |
| Access date | 2016-10-11T12:50:16Z | |
| Available date | 2016-10-11T12:50:16Z | |
| Document date | 2012 | |
| Citation | ROCHA, Adalgiza da Silva; et al. Drug and Multidrug Resistance among Mycobacterium leprae Isolates from Brazilian Relapsed Leprosy Patients. Journal of Clinical Microbiology, v.50, n.6, p.1912-1917, June 2012. | pt_BR |
| ISSN | 0095-1137 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16181 | |
| Language | eng | pt_BR |
| Publisher | American Society for Microbiology | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Hanseníase | pt_BR |
| Subject in Portuguese | Resistência a medicanmentos | pt_BR |
| Subject in Portuguese | Pacientes | pt_BR |
| Subject in Portuguese | Brasil | pt_BR |
| Title | Drug and multidrug resistance among Mycobacterium leprae isolates from Brazilian relapsed leprosy patients | pt_BR |
| Type | Article | |
| DOI | 10.1128/JCM.06561-11 | |
| Abstract | Skin biopsy samples from 145 relapse leprosy cases and from five different regions in Brazil were submitted for sequence analysis of part of the genes associated with Mycobacterium leprae drug resistance. Single nucleotide polymorphisms (SNPs) in these genes were observed in M. leprae from 4 out of 92 cases with positive amplification (4.3%) and included a case with a mutation in rpoB only, another sample with SNPs in both folP1 and rpoB, and two cases showing mutations in folP1, rpoB, and gyrA, suggesting the existence of multidrug resistance (MDR). The nature of the mutations was as reported in earlier studies, being CCC to CGC in codon 55 in folP (Pro to Arg), while in the case of rpoB, all mutations occurred at codon 531, with two being a transition of TCG to ATG (Ser to Met), one TCG to TTC (Ser to Phe), and one TCG to TTG (Ser to Leu). The two cases with mutations in gyrA changed from GCA to GTA (Ala to Val) in codon 91. The median time from cure to relapse diagnosis was 9.45 years but was significantly shorter in patients with mutations (3.26 years; P = 0.0038). More than 70% of the relapses were multibacillary, including three of the mutation-carrying cases; one MDR relapse patient was paucibacillary. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Alfredo da Matta de Dermatologia Tropical (FUAM). Manaus, AM, Brasil. | pt_BR |
| Affilliation | Santa Casa de Misericórdia (EMESCAM). Seção de Dermatologia. Vitória, ES, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Pará. Laboratório de Dermato-imunologia. Unidade de Referência Marcello Candia. Marituba, PA, Brasil. | pt_BR |
| Affilliation | Centro de Referência em Dermatologia Sanitária Dr. Lebanon (CDERM). Fortaleza, CE, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Saúde Pública. Hospital Universitário Clementino Fraga Filho. Centro de Treinamento em Dermatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | National Institute of Infectious Diseases. Leprosy Research Center. Tokyo, Japan. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hanseníase. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Saúde Pública. Hospital Universitário Clementino Fraga Filho. Centro de Treinamento em Dermatologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Multidrug Resistance | pt_BR |
| Subject | Mycobacterium leprae | pt_BR |
| Subject | Leprosy Patients | pt_BR |
| Subject | Brasil | pt_BR |
| e-ISSN | 1098-660X | |
