| Author | Silva, Marcio Roberto | |
| Author | Guimarães, Mark Drew Crosland | |
| Author | Oliveira, Vania Maria de | |
| Author | Moreira, Aline dos Santos | |
| Author | Costa, Ronaldo Rodrigues da | |
| Author | Abi-Zaid, Kelly Cristina Ferreira | |
| Author | Rocha, Adalgiza da Silva | |
| Author | Suffys, Philip Noel | |
| Access date | 2016-10-11T15:09:13Z | |
| Available date | 2016-10-11T15:09:13Z | |
| Document date | 2011 | |
| Citation | SILVA, Marcio Roberto; et al. Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1. Mem Inst Oswaldo Cruz, v.106, n.1, p.9-15, Feb. 2011. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16188 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Mycobacterium tuberculosis | pt_BR |
| Subject in Portuguese | Mycobacterium bovis | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | baciloscopia | pt_BR |
| Subject in Portuguese | Dados de seqüência molecular | pt_BR |
| Title | Identification of Mycobacterium tuberculosis complex based on amplification and sequencing of the oxyR pseudogene from stored Ziehl-Neelsen-stained sputum smears in Brazil1 | pt_BR |
| Type | Article | |
| Abstract | A cross-sectional analysis of stored Ziehl-Neelsen (ZN)-stained sputum smear slides (SSS) obtained from two public tuberculosis referral laboratories located in Juiz de Fora, Minas Gerais, was carried out to distinguish Mycobacterium bovis from other members of the Mycobacterium tuberculosis complex (MTC). A two-step approach was used to distinguish M. bovis from other members of MTC: (i) oxyR pseudogene amplification to detect MTC and, subsequently, (ii) allele-specific sequencing based on the polymorphism at position 285 of this gene. The oxyR pseudogene was successfully amplified in 100 of 177 (56.5%) SSS available from 99 individuals. No molecular profile of M. bovis was found. Multivariate analysis indicated that acid-fast bacilli (AFB) results and the source laboratory were associated (p < 0.05) with oxyR pseudogene amplification. SSS that were AFB++ SSS showed more oxyR pseudogene amplification than those with AFB0, possibly due to the amount of DNA. One of the two source laboratories presented a greater chance of oxyR pseudogene amplification, suggesting that differences in sputum conservation between laboratories could have influenced the preservation of DNA. This study provides evidence that stored ZN-SSS can be used for the molecular detection of MTC. | pt_BR |
| Affilliation | Ministério da Agricultura, Pecuária e Abastecimento. EMBRAPA Gado de Leite. Juiz de Fora, MG, Brasil / Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Programa de Pós-Graduação em Saúde Pública. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Minas Gerais. Faculdade de Medicina. Departamento de Medicina Preventiva e Social. Programa de Pós-Graduação em Saúde Pública. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Ministério da Agricultura, Pecuária e Abastecimento. EMBRAPA Gado de Leite. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Juiz de Fora. Hospital Universitário. Juiz de Fora, MG, Brasil / Fundação Hospitalar do Estado de Minas. Hospital João Penido. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Fundação Hospitalar do Estado de Minas. Hospital João Penido. Juiz de Fora, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicada a Micobactérias. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | oxyR pseudogene | pt_BR |
| Subject | polymerase chain reaction | pt_BR |
| Subject | sputum smear | pt_BR |
| Subject | Mycobacterium tuberculosis | pt_BR |
| Subject | Mycobacterium bovis | pt_BR |
| Subject | molecular sequence data | pt_BR |
| e-ISSN | 1678-8060 | |
