| Author | Oliveira Jr, F. O. R. | |
| Author | Alves, C. R. | |
| Author | Silva, F. Souza | |
| Author | Calvet, C. M. | |
| Author | Côrtes, L. M. C. | |
| Author | Gonzalez, M. S. | |
| Author | Toma, L. | |
| Author | Bouças, R. I. | |
| Author | Nader, H. B. | |
| Author | Pereira, M .C. S. | |
| Access date | 2016-10-13T13:27:52Z | |
| Available date | 2016-10-13T13:27:52Z | |
| Document date | 2012 | |
| Citation | OLIVEIRA JR, F. O. R. et al. Trypanosoma cruzi heparin-binding proteins mediate the adherence of epimastigotes to the midgut epithelial cells of Rhodnius prolixus. Parasitology, v.139, v.6, p.735-743, May 2012. | pt_BR |
| ISSN | 0031-1820 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16211 | |
| Language | eng | pt_BR |
| Publisher | Cambridge University Press | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Trypanosoma cruzi | pt_BR |
| Subject in Portuguese | Proteínas de ligação à heparina | pt_BR |
| Subject in Portuguese | Rhodnius prolixus | pt_BR |
| Subject in Portuguese | Interação parasito-vetor | pt_BR |
| Title | Trypanosoma cruzi heparin-binding proteins mediate the adherence of epimastigotes to the midgut epithelial cells of Rhodnius prolixus | pt_BR |
| Type | Article | |
| DOI | 10.1017/S0031182011002344 | |
| Abstract | Heparin-binding proteins (HBPs) have been demonstrated in both infective forms of Trypanosoma cruzi and are involved in the recognition and invasion of mammalian cells. In this study, we evaluated the potential biological function of these proteins during the parasite-vector interaction. HBPs, with molecular masses of 65·8 kDa and 59 kDa, were isolated from epimastigotes by heparin affinity chromatography and identified by biotin-conjugated sulfated glycosaminoglycans (GAGs). Surface plasmon resonance biosensor analysis demonstrated stable receptor-ligand binding based on the association and dissociation values. Pre-incubation of epimastigotes with GAGs led to an inhibition of parasite binding to immobilized heparin. Competition assays were performed to evaluate the role of the HBP-GAG interaction in the recognition and adhesion of epimastigotes to midgut epithelial cells of Rhodnius prolixus. Epithelial cells pre-incubated with HBPs yielded a 3·8-fold inhibition in the adhesion of epimastigotes. The pre-treatment of epimastigotes with heparin, heparan sulfate and chondroitin sulfate significantly inhibited parasite adhesion to midgut epithelial cells, which was confirmed by scanning electron microscopy. We provide evidence that heparin-binding proteins are found on the surface of T. cruzi epimastigotes and demonstrate their key role in the recognition of sulfated GAGs on the surface of midgut epithelial cells of the insect vector. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Geral. Nterói, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo - UNIFESP. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo - UNIFESP. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Universidade Federal de São Paulo - UNIFESP. Departamento de Bioquímica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ultra-estrutura Celular. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | heparin-binding proteins | pt_BR |
| Subject | Trypanosoma cruzi | pt_BR |
| Subject | epimastigotes | pt_BR |
| Subject | Rhodnius prolixus parasite-vector interaction | pt_BR |
| e-ISSN | 1469-8161 | |
| Embargo date | 2030-01-01 | |
