| Author | Pinho-Ribeiro, Felipe A. | |
| Author | Zarpelon, Ana Carla | |
| Author | Mizokami, Sandra S. | |
| Author | Borghi, Sergio M. | |
| Author | Bordignon, Juliano | |
| Author | Silva, Rangel L. | |
| Author | Cunha, Thiago M. | |
| Author | Alves-Filho, Jose C. | |
| Author | Cunha, Fernando Q. | |
| Author | Casagrande, Rubia | |
| Author | Verri Jr, Waldiceu Aparecido | |
| Access date | 2016-10-13T16:39:00Z | |
| Available date | 2016-10-13T16:39:00Z | |
| Document date | 2016 | |
| Citation | PINHO-RIBEIRO, F. A. et al. The citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-κB activation. Journal of Nutritional Biochemistry, v. 33, p. 8-14, 2016. | pt_BR |
| ISSN | 0955-2863 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16223 | |
| Sponsorship | Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brazil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), São Paulo Research Foundation under grant agreements n° 2011/19670-0 (Thematic project) and 2013/08216-2 (Center for Research in Inflammatory Disease), Ministério da Ciência, Tecnologia e Inovação (MCTI), SETI, Fundação Araucária, and Parana State Government | pt_BR |
| Language | eng | pt_BR |
| Publisher | Bernhard Hennig | pt_BR |
| Rights | restricted access | pt_BR |
| Title | The citrus flavonone naringenin reduces lipopolysaccharide-induced inflammatory pain and leukocyte recruitment by inhibiting NF-κB activation | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.jnutbio.2016.03.013 | pt_BR |
| Abstract | Lipopolysaccharide (LPS) is the major structural component of Gram-negative bacteria cell wall and a highly pro-inflammatory toxin. Naringenin is found in Citrus fruits and exhibits antioxidant and anti-inflammatory properties through inhibition of NF-κB activation but its effects in LPS-induced inflammatory pain and leukocyte recruitment were not investigated yet. We investigated the effects of naringenin in mechanical hyperalgesia, thermal hyperalgesia and leukocyte recruitment induced by intraplantar injection of LPS in mice. We found that naringenin reduced hyperalgesia to mechanical and thermal stimuli, myeloperoxidase (MPO, a neutrophil and macrophage marker) and N-acetyl-β-D-glucosaminidase (NAG, a macrophage marker) activities, oxidative stress and cytokine (TNF-α, IL-1β, IL-6, and IL-12) production in the paw skin. In the peritoneal cavity, naringenin reduced neutrophil and mononuclear cell recruitment, and abrogated MPO and NAG activity, cytokine and superoxide anion production, and lipid peroxidation. In vitro, pre-treatment with naringenin inhibited superoxide anion and cytokine (TNF-α, IL-1β, IL-6, and IL-12) production by LPS-stimulated RAW 264.7 macrophages. Finally, we demonstrated that naringenin inhibited NF-κB activation in vitro and in vivo. Therefore, naringenin is a promising compound to treat LPS-induced inflammatory pain and leukocyte recruitment. | pt_BR |
| Affilliation | Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Centro de Ciências Biológicas, Londrina, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Centro de Ciências Biológicas, Londrina, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Londrina. Departamento de Ciências Patológicas. Centro de Ciências Biológicas, Londrina, PR, Brasil. | pt_BR |
| Affilliation | Universidade Estadual de Londrina. Centro de Ciências Biológicas. Departamento de Ciências Patológicas. Londrina, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Affilliation | University of São Paulo. Ribeirão Preto Medical School. Department of Pharmacology, Ribeirão Preto, SP, Brasil. | En |
| Affilliation | University of São Paulo. Ribeirão Preto Medical School. Department of Pharmacology, Ribeirão Preto, SP, Brasil. | En |
| Affilliation | University of São Paulo. Ribeirão Preto Medical School. Department of Pharmacology, Ribeirão Preto, SP, Brasil. | En |
| Affilliation | University of São Paulo. Ribeirão Preto Medical School. Department of Pharmacology, Ribeirão Preto, SP, Brasil. | En |
| Affilliation | Universidade Estadual de Londrina. Centro de Ciências da Saúde. Departamento de Ciências Farmacêuticas, Londrina, PR, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. | pt_BR |
| Subject | Inflammatory pain | pt_BR |
| Subject | Cytokines | pt_BR |
| Subject | Oxidative stress | pt_BR |
| Subject | Leukocyte recruitment | pt_BR |
| Subject | NF-κB | pt_BR |
| DeCS | Citocinas | pt_BR |
| DeCS | Estresse Oxidativo | pt_BR |
