| Author | Pereira, Daniela de Pita | |
| Author | Souza, Getúlio D. | |
| Author | Pereira, Thaís de Araújo | |
| Author | Zwetsch, Adriana | |
| Author | Britto, Constança | |
| Author | Rangel, Elizabeth F. | |
| Access date | 2016-10-13T17:47:59Z | |
| Available date | 2016-10-13T17:47:59Z | |
| Document date | 2011 | |
| Citation | PEREIRA, Daniela de Pita; et al. Lutzomyia (Pintomyia) fischeri (Diptera: Psychodidae: Phlebotominae), a probable vector of American Cutaneous Leishmaniasis: Detection of natural infection by Leishmania (Viannia) DNA in specimens from the municipality of Porto Alegre (RS), Brazil, using multiplex PCR assay. Acta Tropica, v.120, n.3, p.273-275, Dec. 2011. | pt_BR |
| ISSN | 0001-706X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16229 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Leishmania | pt_BR |
| Subject in Portuguese | Lutzomyia | pt_BR |
| Subject in Portuguese | Infecção natural | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Porto Alegre | pt_BR |
| Title | Lutzomyia (Pintomyia) fischeri (Diptera: Psychodidae: Phlebotominae), a probable vector of American cutaneous leishmaniasis: detection of natural infection by Leishmania (Viannia) DNA in specimens from the municipality of Porto Alegre (RS), Brazil, using multiplex PCR assay | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.actatropica.2011.09.004 | |
| Abstract | In order to determine natural Leishmania (Viannia) infection in Lutzomyia (Pintomyia) fischeri, a multiplex PCR methodology coupled to non-isotopic hybridization was adopted for the analysis of sand fly samples collected by CDC light traps in an endemic area of American Cutaneous Leishmaniasis (ACL) in the periurban region of the municipality of Porto Alegre, Rio Grande do Sul State, Brazil. We analyzed by PCR methodology 560 specimens of Lutzomyia (Pintomyia) fischeri (520 females and 40 males). The wild sand flies were grouped into 56 pools (52 females and 4 males) of 10 each, and positive results were detected in 2 of the 52 female pools, representing a minimum infection rate of 0.38% based on the presence of at least 1 infected insect in the pool. This result associated with some local evidence such as anthopophily, spatial distribution in accordance with the transmission area and human case incidence, suggests that L. (P.)fischeri may be considered as a secondary vector of ACL in the studied locality. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Secretaria Estadual de Saúde do Rio Grande do Sul. Fundação Estadual de Produção e Pesquisa em Saúde. Laboratório Central de Pública do Rio Grande do Sul. Instituto de Pesquisas Biológicas. Seção de Reservatórios e Vetores. Porto Alegre, RS, Brasil / Secretaria Municipal de Saúde de Porto Alegre. Coordenadoria Geral de Vigilância em Saúde. Núcleo de Vigilância de Roedores e Vetores. Porto Alegre, RS, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Transmissores de Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Leishmania (Viannia) | pt_BR |
| Subject | Lutzomyia (P.) fischeri | pt_BR |
| Subject | PCR multiplex | pt_BR |
| Subject | Natural infection | pt_BR |
| Subject | Porto Alegre, Brazil | pt_BR |
| e-ISSN | 1873-6254 | |
