| Author | Pylro, Victor Satler | |
| Author | Morais, Daniel Kumazawa | |
| Author | Kalks, Karlos Henrique Martins | |
| Author | Roesch, Luiz Fernando Wurdig | |
| Author | Hirsch, Penny R. | |
| Author | Tótola, Marcos Rogério | |
| Author | Yotoko, Karla | |
| Access date | 2016-10-18T13:34:58Z | |
| Available date | 2016-10-18T13:34:58Z | |
| Document date | 2016 | |
| Citation | PYLRO, Victor Satler et al. Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases. Journal of Microbiological Methods, v. 126, p. 18-23, Jul. 2016. | pt_BR |
| ISSN | 0167-7012 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16269 | |
| Language | eng | pt_BR |
| Rights | restricted access | pt_BR |
| Title | Misguided phylogenetic comparisons using DGGE excised bands may contaminate public sequence databases | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.mimet.2016.04.012 | |
| Abstract | Controversy surrounding bacterial phylogenies has become one of the most important challenges for microbial ecology. Comparative analyses with nucleotide databases and phylogenetic reconstruction of the amplified 16S rRNA genes from DGGE (Denaturing Gradient Gel Electrophoresis) excised bands have been used by several researchers for the identification of organisms in complex samples. Here, we individually analyzed DGGE-excised 16S rRNA gene bands from 10 certified bacterial strains of different species, and demonstrated that this kind of approach can deliver erroneous outcomes to researchers, besides causing/emphasizing errors in public databases. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Pampa. São Gabriel, RS, Brasil. | pt_BR |
| Affilliation | AgroEcology Department, Rothamsted Research. Harpenden, Herts. United Kingdom, UK. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Microbiologia. Viçosa, MG, Brasil. | pt_BR |
| Affilliation | Universidade Federal de Viçosa. Departamento de Biologia. Viçosa, MG, Brasil. | pt_BR |
| Subject | 16S rRNA | pt_BR |
| Subject | PCR-DGGE bacteria phylogeny | pt_BR |
| Subject | Public databases | pt_BR |
| Embargo date | 2030-12-31 | |
