| Author | Losada-Barragán, Monica | |
| Author | Cavalcanti, Amanda | |
| Author | Umaña-Pérez, Adriana | |
| Author | Porrozzi, Renato | |
| Author | Cuervo-Escobar, Sergio | |
| Author | Vallejo, Andrés Felipe | |
| Author | Sánchez-Gómez, Myriam | |
| Author | Cuervo, Patricia | |
| Access date | 2016-12-08T15:37:02Z | |
| Available date | 2016-12-08T15:37:02Z | |
| Document date | 2016 | |
| Citation | LOSADA-BARRAGÁN, Monica; et al. Detection and quantification of Leishmania infantum in naturally and experimentally infected animal samples. Veterinary Parasitology, v.226, p.57-64, 2016. | pt_BR |
| ISSN | 0304-4017 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16488 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Leishmaniose | pt_BR |
| Subject in Portuguese | Reação em Cadeia da Polimerase | pt_BR |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Amostras de animais infectados | pt_BR |
| Title | Detection and quantification of Leishmania infantum in naturally and experimentally infected animal samples | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.vetpar.2016.05.022 | |
| Abstract | Leishmania infantum is one of the causative agents of visceral leishmaniasis (VL). VL is the most severe form of leishmaniasis and can be fatal if it is not properly treated. Although several PCR works are intended to detect L. infantum, in silico analysis of available primers and/or primer-probes reveals potential cross species amplification. Here, a TaqMan-based quantitative real time PCR (qPCR) assay was developed for specific detection and quantitation of L. infantum in tissue samples from experimentally or naturally infected animals, mice or dogs, respectively. For this assay, primers and probes were designed for the kinetoplast minicircle DNA of L. infantum. The qPCR assay achieved a detection limit of 0.01pg of parasite DNA, and allowed specific amplification of L. infantum in both asymptomatic and symptomatic naturally infected dogs with inter-assay variation coefficients between 0.05-0.11. There was no cross amplification with dog DNA or with L. braziliensis, L. donovani, L. major, L. tropica or Trypanosoma cruzi. In addition, our assay detected a significantly higher parasite load in symptomatic than in the asymptomatic animals (p<0.0001). We believe this approach will be a valuable tool for the specific detection of L. infantum in regions of sympatric transmission of VL-causing parasites. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad Nacional de Colombia. Sede Bogotá. Facultad de Ciencias, Departamento de Química. Grupo de Investigación en Hormonas. Bogotá, Colombia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad Nacional de Colombia. Sede Bogotá. Facultad de Ciencias, Departamento de Química. Grupo de Investigación en Hormonas. Bogotá, Colombia. | pt_BR |
| Affilliation | Universidad Nacional de Colombia. Sede Bogotá. Facultad de Ciencias, Departamento de Química. Grupo de Investigación en Hormonas. Bogotá, Colombia. | pt_BR |
| Affilliation | Universidad Nacional de Colombia. Sede Bogotá. Facultad de Ciencias, Departamento de Química. Grupo de Investigación en Hormonas. Bogotá, Colombia. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Leishmaniose. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Leishmania infantum | pt_BR |
| Subject | visceral leishmaniasis | pt_BR |
| Subject | quantitative real time PCR | pt_BR |
| Subject | infected animal samples | pt_BR |
| e-ISSN | 1873-2550 | |
| Embargo date | 2030-01-01 | |
