| Author | Victoria, M. | |
| Author | Tort, L. F. L. | |
| Author | Lizasoain, A. | |
| Author | García, M. | |
| Author | Castells, M. | |
| Author | Berois, M. | |
| Author | Divizia, M. | |
| Author | Leite, J. P. G. | |
| Author | Miagostovich, M. P. | |
| Author | Cristina, J. | |
| Author | Colina, R. | |
| Access date | 2016-12-26T14:05:43Z | |
| Available date | 2016-12-26T14:05:43Z | |
| Document date | 2016 | |
| Citation | VICTORIA, M. et al. Norovirus molecular detection in Uruguayan sewage samples reveals a high genetic diversity and GII.4 variant replacement a long time . Journal of Applied Microbiology, v.120, p.1427--1435, 2016. | pt_BR |
| ISSN | 1364-5072 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16559 | |
| Language | eng | pt_BR |
| Publisher | The Society for Applied Microbiology | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Norovirus | pt_BR |
| Subject in Portuguese | Uruguai | pt_BR |
| Subject in Portuguese | Epidemiologia molecular | pt_BR |
| Subject in Portuguese | Diversidade genética | pt_BR |
| Subject in Portuguese | Gastroenterite | pt_BR |
| Title | Norovirus molecular detection in Uruguayan sewage samples reveals a high genetic diversity and GII.4 variant replacement along time | pt_BR |
| Type | Article | |
| DOI | 10.1111/jam.13058 | |
| Abstract | Aims: To determine the prevalence and molecular epidemiology of norovirus (NoV) genogroup I (GI) and GII in Uruguay. Methods and Results: One hundred and sixteen sewage samples were collected in six cities (Bella Uni on, Salto, Paysand u, Fray Bentos, Melo and Treinta y Tres) from March 2011 to April 2013, viruses were concentrated by ultracentrifugation and NoV studies were performed by semi-nested RT-PCR (partial capsid region). NoV were detected in samples from all the cities and detected in 72% (84/116) of the samples with nine of them belonging to GI, 48 to GII and 27 to both genogroups. Remarkably, a high genetic diversity was identified: GII.2 (n = 13), GII.4 (n = 13), GI.1 (n = 5), GI.4 (n = 5), GI.8 (n = 4), GII.13 (n = 4), GII.1 (n = 3), GII.6 (n = 3), GI.3 (n = 1), GI.5 (n = 1), GI.6 (n = 1), GII.3 (n = 1), GII.17 (n = 1). Interestingly, a complete replacement of GII.4 New Orleans 2009 by GII.4 Sydney 2012 variants during 2012 was evidenced. Conclusion: This study reveals a high circulation of different NoV GI and GII genotypes in sewage evidencing a replacement of GII.4 variants. Significance and Impact of Study: This approach can be used as an indicator of the presence of a new GII.4 variant which can originate an increase in acute gastroenteritis outbreaks worldwide. | pt_BR |
| Affilliation | Universidad de la República. CENUR Litoral Norte. Department of Biological Sciences. Laboratory of Molecular Virology. Salto, Uruguay. | pt_BR |
| Affilliation | Universidad de la República. CENUR Litoral Norte. Department of Biological Sciences. Laboratory of Molecular Virology. Salto, Uruguay. | pt_BR |
| Affilliation | Universidad de la República. CENUR Litoral Norte. Department of Biological Sciences. Laboratory of Molecular Virology. Salto, Uruguay. | pt_BR |
| Affilliation | Universidad de la República. CENUR Litoral Norte. Department of Biological Sciences. Laboratory of Molecular Virology. Salto, Uruguay. | pt_BR |
| Affilliation | Universidad de la República. School of Sciences. Virology section. Montevideo, Uruguay. | pt_BR |
| Affilliation | Tor Vergata University. Department of Experimental Medicine and Surgery. Laboratory of Environmental Virology. Rome, Italy. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad de la República. School of Sciences. Nuclear Investigation Center. Laboratory of Molecular Virology. Montevideo, Uruguay. | pt_BR |
| Affilliation | Universidad de la República. CENUR Litoral Norte. Department of Biological Sciences. Laboratory of Molecular Virology. Salto, Uruguay. | pt_BR |
| Subject | moleuclar epidemiology | pt_BR |
| Subject | norovirus | pt_BR |
| Subject | Uruguay | pt_BR |
| Subject | acute gastroenteritis | pt_BR |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 10 Redução das desigualdades | |
