| Author | Melgaço, Fabiana Gil | |
| Author | Victoria, Matias | |
| Author | Corrêa, Adriana Abreu | |
| Author | Ganime, Ana Carolina | |
| Author | Malta, Fábio Correia | |
| Author | Brandão, Marcelo Luiz Lima | |
| Author | Medeiros, Valéria de Mello | |
| Author | Rosas, Carla de Oliveira | |
| Author | Bricio, Silvia Maria Lopes | |
| Author | Miagostovich, Marize Pereira | |
| Access date | 2017-01-01T16:48:18Z | |
| Available date | 2017-01-01T16:48:18Z | |
| Document date | 2016 | |
| Citation | MELGAÇO, Fabiana Gil; et al. Virus recovering from strawberries: Evalaution of a skimmed milk organic flocculation method for assessment of microbiological contamination, International Journal of Food Microbiology, v.217, p.14-19, Oct. 2016. | pt_BR |
| ISSN | 0168-1605 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16615 | |
| Language | eng | pt_BR |
| Publisher | Elsevier | pt_BR |
| Rights | restricted access | |
| Subject in Portuguese | Transmissão por alimentos | pt_BR |
| Subject in Portuguese | Leite desnatado | pt_BR |
| Subject in Portuguese | Baixo custo | pt_BR |
| Subject in Portuguese | Vírus entéricos | pt_BR |
| Subject in Portuguese | método de floculação | pt_BR |
| Title | Virus recovering from strawberries: Evaluation of a skimmed milk organic flocculation method for assessment of microbiological contamination | pt_BR |
| Type | Article | |
| DOI | 10.1016/j.ijfoodmicro.2015.10.005 | |
| Abstract | Skimmed milk organic flocculation method was adapted, optimized and compared with polyethylene glycol (PEG) precipitation and filtration methods for recovering viruses from a strawberry matrix. Spiking experiments with norovirus genogroup II genotype 4 (NoV GII.4) and murine norovirus 1 (MNV-1) demonstrated that the organic flocculation method associated with a glycine elution buffer, filter bag and cetyltrimethylammonium bromide (CTAB) showed a recovery percentage of 2.5 and 32 times higher than PEG precipitation and filtration methodologies for NoV recovering. Furthermore, this method was used for investigating NoV and human adenoviruses (HAdVs) in 90 samples of fresh strawberries commercialized in Rio de Janeiro markets. NoV GI and GII were not detected in those samples and MNV-1, used as internal process control (IPC), was recovered in 95.5% (86) of them. HAdVs were detected in 18 (20.0%) samples and characterized by nucleotide sequencing as Human Mastadenovirus specie F and as type specie HAdV-2. Bacterial analysis did not detect Salmonella spp. and Listeria monocytogenes, however, 3.3% of fecal coliforms were detected in those samples. These results indicate the organic flocculation method as an alternative for recovering enteric viruses from strawberries, emphasizing a need for virus surveillance in food matrices. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambeintal. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidad de la ´Republica. CENUR Noroeste. Regional Norte. Laboratório de Virologia Moleucar. Salto, Uruguay. | pt_BR |
| Affilliation | Universidade Federal Fluminense. Laboratório de Virologia. Niterói, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambeintal. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Faculdade de Farmácia. Laboratório de Microbiologia e Parasitologia. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Alimentos e Contaminantes. Setor de Alimentos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Alimentos e Contaminantes. Setor de Alimentos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Alimentos e Contaminantes. Setor de Alimentos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Nacional de Controle de Qualidade em Saúde. Departamento de Microbiologia. Laboratório de Alimentos e Contaminantes. Setor de Alimentos. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambeintal. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Subject | Foodborne | pt_BR |
| Subject | Skimmed milk | pt_BR |
| Subject | Low cost | pt_BR |
| Subject | Strawberry | pt_BR |
| Subject | flocculation method | pt_BR |
| Subject | Enteric virus | pt_BR |
| Embargo date | 2030-01-01 | |
| xmlui.metadata.dc.subject.ods | 02 Fome zero e agricultura sustentável | |
