| Author | Souza, Fernando Almeida | |
| Author | Taniwaki, Noemi Nosomi | |
| Author | Amaral, Ana Cláudia Fernandes | |
| Author | Souza, Celeste da Silva Freitas de | |
| Author | Calabrese, Kátia da Silva | |
| Author | Silva, Ana Lúcia Abreu | |
| Access date | 2017-01-03T11:26:34Z | |
| Available date | 2017-01-03T11:26:34Z | |
| Document date | 2016 | |
| Citation | SOUZA, Fernando Almeida; et al. Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment. Evidence-Based Complementary and Alternative Medicine, Volume 2016, Article ID 5063540, 9p, 2016. | pt_BR |
| ISSN | 1741-427X | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16633 | |
| Language | eng | pt_BR |
| Publisher | Hindawi Publishing Corporation | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Leishmania infantum | pt_BR |
| Subject in Portuguese | Morinda citrifolia Linn | pt_BR |
| Subject in Portuguese | Suco de fruta de M. citrifolia | pt_BR |
| Subject in Portuguese | Tratamento | pt_BR |
| Title | Ultrastructural Changes and Death of Leishmania infantum Promastigotes Induced by Morinda citrifolia Linn. Fruit (Noni) Juice Treatment | pt_BR |
| Type | Article | |
| DOI | 10.1155/2016/5063540 | |
| Abstract | The search for new treatments against leishmaniasis has increased due to high frequency of drug resistance registered in endemics areas, side effects, and complications caused by coinfection with HIV. Morinda citrifolia Linn., commonly known as Noni, has a rich chemical composition and various therapeutic effects have been described in the literature. Studies have shown the leishmanicidal activity of M. citrifolia; however, its action on the parasite has not yet been elucidated. In this work, we analyzed leishmanicidal activity and ultrastructural changes in Leishmania infantum promastigotes caused by M. citrifolia fruit juice treatment. M. citrifolia fruit extract showed a yield of 6.31% and high performance liquid chromatography identified phenolic and aromatic compounds as the major constituents. IC50 values were 260.5 µg/mL for promastigotes and 201.3 µg/mL for intracellular amastigotes of L. infantum treated with M. citrifolia. Cytotoxicity assay with J774.G8 macrophages showed that M. citrifolia fruit juice was not toxic up to 2 mg/mL. Transmission electron microscopy showed cytoplasmic vacuolization, lipid inclusion, increased exocytosis activity, and autophagosome-like vesicles in L. infantum promastigotes treated with M. citrifolia fruit juice. M. citrifolia fruit juice was active against L. infantum in the in vitro model used here causing ultrastructural changes and has a future potential for treatment against leishmaniasis. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ. Brasil / Universidade Estadual do Maranhão. Departamento de Patologia. São Luís, MA, Brasil. | pt_BR |
| Affilliation | Instituto Adolfo Lutz. Unidade de Microscopia Eletrônica. São Paulo, SP, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Farmanguinhos. Laboratório de Plantas Medicinais e Derivados. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Universidade Estadual do Maranhão. Departamento de Patologia. São Luís, MA, Brasil. | pt_BR |
| Subject | Leishmania infantum promastigotes | pt_BR |
| Subject | Morinda citrifolia Linn. Fruit (Noni) | pt_BR |
| Subject | M. citrifolia fruit juice | pt_BR |
| Subject | Treatment | pt_BR |
| e-ISSN | 1741-4288 | |
