| Author | Cárdenas, Melissa Q. | |
| Author | Lanfredi, Reinalda M. | |
| Author | Menezes, Aleksandra Oliveira | |
| Access date | 2017-01-16T13:49:31Z | |
| Available date | 2017-01-16T13:49:31Z | |
| Document date | 2012 | |
| Citation | CÁRDENAS, Melissa Q.; LANFREDI, Reinalda M.; MENEZES, Aleksandra Oliveira. Cytochemical Analysis of the Body Wall of the Flounder Parasite Procamallanus (Spirocamallanus) halitrophus (Nematoda: Camallanidae) . Comp. Parasitol., v.79, n.2, p.173-181, 2012. | pt_BR |
| ISSN | 1525-2647 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16842 | |
| Language | eng | pt_BR |
| Publisher | Helminthological Society of Washington | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Ultraestrutura | pt_BR |
| Subject in Portuguese | Histocitoquímica | pt_BR |
| Subject in Portuguese | Parasitas intestinais | pt_BR |
| Title | Cytochemical Analysis of the Body Wall of the Flounder Parasite Procamallanus (Spirocamallanus) halitrophus (Nematoda: Camallanidae) | pt_BR |
| Type | Article | |
| DOI | 10.1654/4565.1 | |
| Abstract | Procamallanus (Spirocamallanus) halitrophus (Fusco and Overstreet, 1978) is an intestinal parasite of the flounders Syacium papillosum and Citharichthys macrops, both of which are native to waters off the coast of the state of Rio de Janeiro, Brazil. With transmission electron microscopy, we observed the body wall which is composed of the cuticle, hypodermis, and somatic musculature. The cuticle of P. (S.) halitrophus is composed of 5 layers: the epicuticle, cortical, median, fibrous, and basal layers. Underlying the cuticle is the hypodermis, a syncytium that contains mitochondria, glycogen granules, vesicles, inclusion bodies, and an endoplasmic reticulum. The region of hypodermal chords contains a nucleus in addition to the other organelles and there is a basal lamina surrounding each muscle cell. The use of imidazolebuffered osmium tetroxide solution revealed the presence of lipids in the epicuticle, the membrane that surrounds each muscle cell, the inclusion bodies, and the endoplasmic reticulum. The phosphotungstic-acid technique revealed basic proteins in the epicuticle and dense bodies. The use of periodic acid-thiosemicarbazide-silver proteinate for carbohydrate detection did not show any reaction products in the cuticle. However, glycogen particles were evident in the hypodermis and muscle cells. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Helmintos Parasitos de Peixes. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Biologia de Helmintos Otto Wucherer. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Universidade Federal do Rio de Janeiro. Campus Macaé. Grupo de Sistemática e Biologia Evolutiva (GSE), Pólo Barreto. Macaé, RJ, Brasil. | pt_BR |
| Subject | Procamallanus (S.) halitrophus | pt_BR |
| Subject | TEM | pt_BR |
| Subject | ultrastructure | pt_BR |
| Subject | cytochemistry | pt_BR |
