| Author | Silva, Allan Peres da | |
| Author | Almeida, Adilson José de | |
| Author | Lampe, Elisabeth | |
| Access date | 2017-01-17T14:31:33Z | |
| Available date | 2017-01-17T14:31:33Z | |
| Document date | 2012 | |
| Citation | SILVA, Allan Peres da; ALMEIDA, Adilson José de; LAMPE, Elisabeth. Genetic diversity of NS3 protease from Brazilian HCV isolates and possible implications for therapy with direct-acting antiviral drugs. Mem Inst Oswaldo Cruz, v.107, n.2, p.254-261, Mar. 2012. | pt_BR |
| ISSN | 0074-0276 | pt_BR |
| URI | https://www.arca.fiocruz.br/handle/icict/16867 | |
| Language | eng | pt_BR |
| Publisher | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz | pt_BR |
| Rights | open access | |
| Subject in Portuguese | Diversidade genética | pt_BR |
| Subject in Portuguese | Vírus da hepatite C | pt_BR |
| Subject in Portuguese | Protease | pt_BR |
| Subject in Portuguese | Agentes antivirais de ação direta | pt_BR |
| Title | Genetic diversity of NS3 protease from Brazilian HCV isolates and possible implications for therapy with direct-acting antiviral drugs | pt_BR |
| Type | Article | |
| Abstract | The hepatitis C virus (HCV) NS3 protease has been one of the molecular targets of new therapeutic approaches.
Its genomic sequence variability in Brazilian HCV isolates is poorly documented. To obtain more information on the
magnitude of its genetic diversity, 114 Brazilian HCV samples were sequenced and analysed together with global
reference sequences. Genetic distance (d) analyses revealed that subtype 1b had a higher degree of heterogeneity
(d = 0.098) than subtypes 1a (d = 0.060) and 3a (d = 0.062). Brazilian isolates of subtype 1b were distributed in
the phylogenetic tree among sequences from other countries, whereas most subtype 1a and 3a sequences clustered
into a single branch. Additional characterisation of subtype 1a in clades 1 and 2 revealed that all but two Brazilian
subtype 1a sequences formed a distinct and strongly supported (approximate likelihood-ratio test = 93) group of
sequences inside clade 1. Moreover, this subcluster inside clade 1 presented an unusual phenotypic characteristic
in relation to the presence of resistance mutations for macrocyclic inhibitors. In particular, the mutation Q80K was
found in the majority of clade 1 sequences, but not in the Brazilian isolates. These data demonstrate that Brazilian
HCV subtypes display a distinct pattern of genetic diversity and reinforce the importance of sequence information
in future therapeutic approaches. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil / Universidade Federal do Estado do Rio de Janeiro. Hospital Universitário Gaffrée Guinle. Rio de Janeiro, RJ, Brasil. | pt_BR |
| Affilliation | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ. Brasil. | pt_BR |
| Subject | genetic diversity | pt_BR |
| Subject | NS3 protease | pt_BR |
| Subject | hepatitis C virus | pt_BR |
| Subject | direct-acting antiviral agents | pt_BR |
| e-ISSN | 1678-8060 | |
| xmlui.metadata.dc.subject.ods | 10 Redução das desigualdades | |
